S1076

SRC, active, GST tagged human

PRECISIO^® Kinase, recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(s): ASV, c-SRC, p60-Src
• NACRES: NA.32
• UNSPSC Code: 12352200
• Specific activity: 83-113 nmol/min·mg
• Assay: ≥70% (SDS-PAGE)
• Recombinant: expressed in E. coli

Properties

• recombinant: expressed in E. coli
• Quality Segment: 200
• product line: PRECISIO^® Kinase
• assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: 83-113 nmol/min·mg
• mol wt: ~83 kDa
• UniProt accession no.: P12931
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... SRC(6714)

Description

General description

SRC proto-oncogene, non-receptor tyrosine kinase (SRC) belongs to the family of non-receptor tyrosine kinases. SRC was originally identified as a transforming protein of the Rous sarcoma virus (RSV) that had enzymatic ability to phosphorylate tyrosine in protein substrates. It contains an amino-terminal domain, conserved Src-homology domains (SH2 and SH3), a tyrosine kinase domain and the carboxyl-terminus. The gene encoding this protein is localized on human chromosome 20q11.23.

Application

SRC, active, GST tagged human has been used in the in vitro kinase assay.

Biochem/physiol Actions

SRC proto-oncogene, non-receptor tyrosine kinase (SRC) phosphorylates membrane proteins and nucleoproteins and activates various intracellular signaling cascades. It is overexpressed and activated in a large number of human malignancies and has been linked to the development of cancer and progression to distant metastases. In addition to increasing cell proliferation, a key role of SRC in cancer seems to be the ability to promote invasion and motility, functions that might contribute to tumor progression.

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable