RAB0001

Human IgG ELISA Kit

for serum, plasma

• Synonym(s): IgG, Immunoglobulin-G
• NACRES: NA.32
• UNSPSC Code: 41116158
• Species reactivity: human
• Storage temp.: −20°C
• Shipped in: wet ice

Properties

• species reactivity: human
• technique(s): ELISA: suitable, capture ELISA: suitable
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Immunoglobulin G (IgG) belongs to immunoglobulin family, and consists of gamma (γ) heavy chain in the constant (C) region. IgG is a widely expressed serum antibody. Monomeric structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weights of 50kDa and 25kDa respectively. The primary structure of this antibody also contains disulfide bonds linking the two heavy chains, linking the heavy and light chains, and are present inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, andγ4, respectively. IgG is produced and released from CD5⁺ B cells, the predominant lymphocytes in the neonatal cell repertoire.[1] Maternal IgG is the only antibody transported across the placenta to the fetus. Maternal IgG passively immunizes the infants.

This ELISA (enzyme-linked immunosorbent assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of a target protein in biological samples such as serum, plasma, cell culture supernatants, urine and/or cell and tissue lysates. This assay employs a specific capture antibody coated on a 96 well plate.

Immunogen

Recombinant Human IGG

Application

Human IgG ELISA Kit has been used to determine the total IgG antibody levels in serum samples of new borns.[2] It has also been used to validate the micro-bead injection spectroscopy (μ-BIS)- lab-on-valve (LOV) method for the determination of serum immunoglobulin G (IgG).[3]

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

• RABWASH4

  20X Wash Buffer (Item B)

  SDS
• RABELADE

  ELISA 5X Assay/Sample Diluent Buffer E (Item E2)

  SDS
• RABTMB3

  ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  SDS
• RABSTOP3

  ELISA Stop Solution (Item I)

  SDS

Safety Information

• pictograms: GHS05
• signalword: Warning
• hcodes: H290
• pcodes: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Storage Class: 12 - Non Combustible Liquids