The amino acid sequence for the two enzymes is the same. The difference between them is that RNase B is a glycoprotein and contains 6 mannose residues and 2 residues of N-acetylglucosamine.
R7884
Ribonuclease B from bovine pancreas
BioReagent, ≥50 Kunitz units/mg protein, ≥80% (SDS-PAGE)
Synonym(s):
RNase B
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About This Item
CAS Number:
EC Number:
UNSPSC Code:
12352204
EC Number:
232-646-6
NACRES:
NA.32
MDL number:
Specific activity:
≥50 Kunitz units/mg protein
Assay:
≥80% (SDS-PAGE)
Biological source:
bovine pancreas
Concentration:
≥60%
Pricing and availability is not currently available.
biological source
bovine pancreas
Quality Segment
product line
BioReagent
assay
≥80% (SDS-PAGE)
form
powder
specific activity
≥50 Kunitz units/mg protein
concentration
≥60%
technique(s)
cell based assay: suitable
input
general use sample(s)
suitability
suitable for molecular biology
application(s)
cell analysis
foreign activity
protease ≤0.001 units/mg solid
storage temp.
−20°C
SMILES string
[nH]1cncc1CC(NC(=O)CCN)C(=O)O
InChI
1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
InChI key
CQOVPNPJLQNMDC-UHFFFAOYSA-N
Application
Biochem/physiol Actions
Native RNase BS generated by subtilisin digestion of native RNase B comprising of amino acid residues 21-124 of RNase B, is sensitive to PNGase F digestion. Intramolecular N-glycans of bovine pancreatic RNase B function like chaperone. RNase B is found to be much faster than RNase A, while RNase A is liable to aggregate during regeneration. The stimulatory effect of Asn-oligosaccharide (which corresponds to the most predominant sugar chain of RNase B) reveals that the N-glycans of RNase B facilitates the transformation of bulky intermediates into folded, compact species.
Packaging
Package size based on protein content
Preparation Note
Purified by affinity chromatography
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This Item | |||
|---|---|---|---|
| description BioReagent, ≥50 Kunitz units/mg protein, ≥80% (SDS-PAGE) | description Type XII-A, ≥90% (SDS-PAGE), 75-125 Kunitz units/mg protein | description Type II-A, ≥60% (SDS-PAGE), >= 60 Kunitz units/mg protein | description Molecular Biology, ≥70 Kunitz units/mg protein, lyophilized |
| technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) - |
| specific activity ≥50 Kunitz units/mg protein | specific activity 75-125 Kunitz units/mg protein | specific activity >= 60 Kunitz units/mg protein | specific activity ≥70 Kunitz units/mg protein |
| assay ≥80% (SDS-PAGE) | assay ≥90% (SDS-PAGE) | assay - | assay - |
| biological source bovine pancreas | biological source bovine pancreas | biological source bovine pancreas | biological source - |
| concentration ≥60% | concentration - | concentration ≥60% (SDS-PAGE) | concentration - |
| application(s) cell analysis | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) - |
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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What is the difference between RNase B, Ribonuclease B from bovine pancreas, Product R7884, and RNase A?
1 answer-
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How should Ribonuclease B from bovine pancreas, Product R7884, be reconstituted?
1 answer-
This product may be reconstituted directly in water at a concentration of 1 mg/mL.
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What is the extinction coefficient of Ribonuclease B from bovine pancreas (RNase B), Product R7884?
1 answer-
The E1% for this enzyme is 8.0 when measured at 280 nm.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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