Theoretically, there is a component that may interfere with fluorescent light microscopy; excitation ~310 nm, emission ~425 nm and (minimally) 530 nm. However, at working dilution of 1:500, the optical interference may be reduced. We recommend choosing fluorescent markers and filter sets that avoid these wavelengths.
R7397
ProtectRNA™ RNase Inhibitor 500× Concentrate
RNase inhibitor for in situ hybridization assays
Synonym(s):
Ribonuclease inhibitor ProtectRNA™
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About This Item
NACRES:
NA.52
UNSPSC Code:
41106300
Pricing and availability is not currently available.
Quality Level
form
liquid
feature
PCR Additives
technique(s)
cDNA synthesis: suitable
storage temp.
2-8°C
General description
Not recommended in systems where other enzymatic activity is required.
ProtectRNA™ ribonuclease(RNase) Inhibitor inhibits most nucleic acid binding enzymes such as RNase and DNase, reverse transcriptase, some RNA and DNA polymerases, protein synthesis enzymes, and several restriction endonucleases. In contrast, ProtectRNA does not interfere with DNA and RNA hybridizations. The action ofProtectRNA as a nuclease inhibitor can be used as an advantage for in situ hybridization. ProtectRNA is useful during all steps of in situ hybridization for tissue RNA protection from RNase degradation. It is recommended to add ProtectRNA to all aqueous solutions used, from as early as the deparaffinization step until the hybridization is completed.
ProtectRNA™ ribonuclease(RNase) Inhibitor inhibits most nucleic acid binding enzymes such as RNase and DNase, reverse transcriptase, some RNA and DNA polymerases, protein synthesis enzymes, and several restriction endonucleases. In contrast, ProtectRNA does not interfere with DNA and RNA hybridizations. The action ofProtectRNA as a nuclease inhibitor can be used as an advantage for in situ hybridization. ProtectRNA is useful during all steps of in situ hybridization for tissue RNA protection from RNase degradation. It is recommended to add ProtectRNA to all aqueous solutions used, from as early as the deparaffinization step until the hybridization is completed.
Application
ProtectRNA™ RNase Inhibitor 500× Concentrate has been used in:
- RNA extraction
- tissue sectioning and staining for laser capture microscopy
- the isolation of blood vessels from hippocampi
A potent inhibitor of most nucleic acid binding enzymes, and thus useful as an RNase inhibitor. Especially useful when performing in situ hybridization. If it is added to all aqueous solutions used, it eliminates the need for special glassware washing and after-wash treatments. The 500x concentrate is economical; 2 ml treats 1,000 ml of solution. Not recommended in systems where other enzymatic activity is required.
ProtectRNA™ RNase Inhibitor 500× Concentrate is an RNase inhibitor.
Features and Benefits
- The 500× concentrate is economical
- Eliminates the need for special dishwashing and“after washing” cleaning and treatments.
Preparation Note
2mL treats 1,000ml of solution.
Other Notes
This product is for R&D use only, not for drug, household, or other uses.
Legal Information
ProtectRNA is a trademark of Sigma-Aldrich Co. LLC
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This Item | |||
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| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 500 |
| form liquid | form - | form liquid | form solution |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| feature PCR Additives | feature - | feature - | feature - |
| technique(s) cDNA synthesis: suitable | technique(s) - | technique(s) - | technique(s) - |
signalword
Warning
hcodes
Hazard Classifications
Flam. Liq. 3 - Met. Corr. 1
Storage Class
3 - Flammable liquids
wgk
WGK 2
flash_point_f
140.0 °F - closed cup
flash_point_c
60 °C - closed cup
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| R7397-30ML | 04061836696313 |
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Does the compund interfere with fluorescent light microscopy?
1 answer-
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Is this compatible with qPCR and LAMP?
1 answer-
This product is not tested for compatibility with qPCR and LAMP applications. Therefore, no references are available.
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Hi , what the inhibitor is ? I would like to do rna electrophoresis in agarose gel. Will this affect RNA move rate?
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The exact identity of the material is proprietary. This material should not affect the migration of RNA on an agarose gel.
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Hello, I am wondering what the concentration in U/uL of this RNAse inhibitor is. U being units. Best, Charles
1 answer-
Unfortunately, this product is not tested to determine the unit activity. The solution is performance tested with a recommended starting dilution factor of 1:500.
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