Yes, R4775 can be used for restriction digestion, as most restriction enzymes, including R4775, have sufficient activity to digest DNA directly in PCR mixtures without the need for the optimal buffer.
R4775
REDExtract-N-Amp™ PCR ReadyMix™
Ready-to-use 2X PCR Master Mix with Loading Dye
REDExtract-N-Amp™ PCR ReadyMix™
Synonym(s):
Direct plant PCR master mix, Direct tissue PCR master mix, PCR master mix with loading dye
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About This Item
NACRES:
NA.55
UNSPSC Code:
41106303
Pricing and availability is not currently available.
Quality Segment
usage
sufficient for 100 reactions, 1.2 mL sufficient for 100 amplifications, sufficient for 1000 reactions, 12 mL sufficient for 1000 amplifications, sufficient for 10000 reactions, 125 mL sufficient for 10000 amplifications
feature
dNTPs included, hotstart
color
red
application(s)
agriculture
shipped in
wet ice
storage temp.
−20°C
General description
The REDExtract-N-Amp™ PCR reaction mix is a PCR Master mix containing buffer, salts, dNTPs, and REDTaq DNA polymerase.[1]
This PCR ReadyMix is intended for use with Sigma′s Extract-N-Amp Plant PCR kit and Extract-N-Amp Tissue PCR Kit. All Extract-N-Amp kits are sufficient for one PCR amplification per extraction. However, where additional PCR reactions are required, this supplemental PCR ReadyMix may be needed. The REDExtract-N-Amp PCR ReadyMix contains an inert tracking dye that allows for convenient direct loading of PCR products onto agarose gels for analysis.
This PCR ReadyMix is intended for use with Sigma′s Extract-N-Amp Plant PCR kit and Extract-N-Amp Tissue PCR Kit. All Extract-N-Amp kits are sufficient for one PCR amplification per extraction. However, where additional PCR reactions are required, this supplemental PCR ReadyMix may be needed. The REDExtract-N-Amp PCR ReadyMix contains an inert tracking dye that allows for convenient direct loading of PCR products onto agarose gels for analysis.
Application
REDExtract-N-Amp™ PCR Master Mix has been used:
REDExtract-N-Amp™ PCR Master Mix is also suitable for colony PCR.
- for the genotyping of fixed zebrafish embryo samples[2]
- in polymerase chain reaction (PCR) amplification of the DNA isolated from lichen family using the REDExtract-N-Amp™ Plant PCR Kit
- in the amplification of DNA isolated from single yeast colonies and fungal spores using REDExtract-N-Amp™ seed PCR kit.
REDExtract-N-Amp™ PCR Master Mix is also suitable for colony PCR.
Features and Benefits
- A Gel-ready- No loading buffer or tracking dye needs to be added. The PCR product is loaded directly into an agarose gel after amplification. The red tracer migrates at approximately the same rate as a 125bp fragment (slightly faster than bromophenol blue).
- Convenience- Hot-start feature in JumpStart™ products allows reaction set up at room temperature, which can be beneficial when working with multiple samples. Ready-mix format reduces setup time.
- Reproducibility- Reduces the risk of contamination from multiple pipet steps and provides consistent performance across both reactions and time.
- High Specificity & Yield- JumpStart Taq DNA polymerase, an antibody inactivated hot-start enzyme, is designed to minimize non-specific amplification while increasing target yield & specificity.
Legal Information
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
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This Item | |||
|---|---|---|---|
| color red | color red | color red | color colorless |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| feature dNTPs included, hotstart | feature dNTPs included, hotstart | feature dNTPs included, hotstart: no, Standard PCR | feature dNTPs included, hotstart, Direct PCR |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| application(s) agriculture | application(s) - | application(s) agriculture | application(s) agriculture |
Storage Class
10 - Combustible liquids
wgk
WGK 3
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
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Can R4775 be used for restriction digestion?
1 answer-
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Please furnish the elongation velocity of the DNA polymerase included in this kit to set the optimal PCR reaction. Thank you.
1 answer-
Velocity is not a factor that is tested or determined for this product. This reagent is a component of several kits, each of which has a specific optimized cycling protocol.
See the link below to review the XNAT kit protocol - note the cycling parameters on page 3.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/141/336/xnatbul.pdfShould another kit product be of interest, please navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Technical Inquiries" under the Products Section, and respond with all the required information so that a member of the Technical Service team can reach out to assist further. Thank you.
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