Please consult the User Guide for all procedures recommended while using this product. If the information is not in the User Guide, we have not tested other conditions out. Link: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/306/054/p9424pis-ms.pdf
P9424
ProteinA-Sepharose™ 4, Fast Flow from Staphylococcus aureus
aqueous ethanol suspension
Synonym(s):
Protein A–Agarose
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About This Item
Form:
aqueous ethanol suspension
Biological source:
Staphylococcus aureus
Pricing and availability is not currently available.
biological source
Staphylococcus aureus
Quality Segment
form
aqueous ethanol suspension
analyte chemical class(es)
proteins (Immunoglobulins of various mammalian species)
extent of labeling
~6 mg per mL
technique(s)
affinity chromatography: suitable
matrix
Sepharose 4B Fast Flow
matrix activation
cyanogen bromide
matrix attachment
amino
matrix spacer
1 atom
capacity
≥30 mg/mL binding capacity (human IgG)
storage temp.
2-8°C
General description
Protein A-Sepharose™ has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.[1]
Application
Protein A-Sepharose is used for affinity chromatography, antibody purification and characterization, immunoaffinity matrices, protein chromatography, protein A, G and L resins, and recombinant protein expression and analysis. Protein A-Sepharose™ has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.
Physical form
Suspension in 20% ethanol
Legal Information
Sepharose is a trademark of Cytiva
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This Item | |||
|---|---|---|---|
| technique(s) affinity chromatography: suitable | technique(s) - | technique(s) affinity chromatography: suitable | technique(s) affinity chromatography: suitable |
| biological source Staphylococcus aureus | biological source Staphylococcus aureus | biological source - | biological source - |
| form aqueous ethanol suspension | form lyophilized powder | form lyophilized powder | form aqueous ethanol suspension |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 100 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
| matrix Sepharose 4B Fast Flow | matrix - | matrix Sepharose CL-4B | matrix Sepharose 4B Fast Flow |
signalword
Warning
hcodes
Hazard Classifications
Flam. Liq. 3
wgk
WGK 3
Storage Class
3 - Flammable liquids
flash_point_f
104.0 °F
flash_point_c
40 °C
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For ChIP-seq experiments, what is the correct procedure to prepare a 50% Protein A–Sepharose bead slurry? Specifically, can the slurry be prepared by pelleting 20 µL of the stock beads at 1,000 g for 30 s, discarding the storage buffer, and resuspending
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