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P8483

Polyamine Supplement (1000×)

liquid, sterile-filtered, BioReagent, suitable for cell culture

Synonym(s):

Polyamine cell culture tested

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About This Item

NACRES:
NA.75
UNSPSC Code:
12352205
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biological source

non-animal source

Quality Segment

sterility

sterile-filtered

product line

BioReagent

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

shipped in

ambient

storage temp.

2-8°C

General description

Polyamine Supplement (1000×) is a general medium supplement specially formulated to boost cell growth for enhanced viral and protein expression. One mL supplement is recommended for 1 liter of medium.

Application

Polyamine Supplement (1000×) has been used with serum-free medium (SFM) to culture human lymphocytes from peripheral blood mononuclear cells (PBMCs).[1] It has also been used in combination with antioxidant supplement (A 1345) to culture zygotes.

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This Item
A1345N6530G9785
form

liquid

form

liquid

form

solution

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

biological source

non-animal source

biological source

-

biological source

-

biological source

-

shipped in

ambient

shipped in

ambient

shipped in

-

shipped in

dry ice

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C


Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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Questions

  1. What polyamines are present in the supplement and at what concentration?

    1 answer
    1. This supplement offers a total amine concentration, at 1000x, of 423 mg/liter. The components and concentration of each are as follows:
      1,4-diamino butane dihydrochloride (known as putrescine dihydrochloride) - 0.005 % or 50 mg/L
      Spermine free base - 0.0048 % or 48 mg/L
      Spermidine free base - 0.0025 % or 25 mg/L
      Ethanolamine - 0.03 % or 300 mg/L

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