P1644

Percoll^®

pH 8.5-9.5 (20 °C)

• Synonym(s): Percoll Density Gradient Media
• UNSPSC Code: 12352207
• NACRES: NA.75
• MDL number: MFCD00131830

Properties

• sterility: aseptically filled
• Quality Segment: 200
• form: colloidal ((Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)) )
• technique(s): cell culture | mammalian: suitable
• color: clear colorless to faint yellow
• pH: 8.5-9.5 (20 °C)
• application(s): hematology; histology
• storage temp.: 2-8°C

Description

General description

Percoll^® is a classic medium for density gradient centrifugation of cells, viruses, and subcellular particles. Percoll consists of colloidal silica particles of 15–30 nm diameter (23% w/w in water), which have been coated with polyvinylpyrrolidone (PVP). The PVP coating renders the product completely non-toxic and ideal for use with biological materials.

Application

Percoll^® has been used in sperm preparation to study the energy requirements of bovine embryos.

Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). It is used in cell separation and organelle isolation.

Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). It is used to establish non-toxic low viscosity, low osmolarity density gradients that can be used to isolate cells, organelles and viruses. It has been used to separate inner and outer membrane vesicles from E. coli. Percoll is isosmotic throughout the gradient, non-toxic, and is easily removed from the purified materials.

Legal Information

Percoll is a registered trademark of Cytiva

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves