P1435

Phosphatase, Acid from sweet potato

ammonium sulfate suspension, ≥10.0 units/mg protein (modified Warburg-Christian)

• Synonym(s): (Orthophosphoric-monoester phosphohydrolase [acid optimum] )
• CAS Number: 9001-77-8
• NACRES: NA.54
• UNSPSC Code: 12352204
• EC Number: 232-630-9
• MDL number: MFCD00131847
• EC Number: 3.1.3.2 (BRENDA, IUBMB)
• eCl@ss: 42010102
• Specific activity: ≥10.0 units/mg protein (modified Warburg-Christian)

Properties

• form: ammonium sulfate suspension
• Quality Segment: 300
• specific activity: ≥10.0 units/mg protein (modified Warburg-Christian)
• secondary activity: ≤9.9 units/mg protein ATPase
• mol wt: 110 kDa
• storage temp.: 2-8°C
• SMILES string: O(CC(O)C)CC#C
• InChI: 1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3
• InChI key: GZCWLCBFPRFLKL-UHFFFAOYSA-N

Description

General description

Acid phosphatase from sweet potato is a phosphomonoesterase, which can appear in multiple molecular forms of similar molecular mass but with different isoelectric points.

Application

Potato acid phosphatase is used as a dephosphorylating reagent in thiochrome assays for the detection of thiamin in biological samples. In this assay, it was shown to be more effective than acid phosphatases from wheat germ or α-amylase.

Sweet potato acid phosphatase has been used in a study as a pre-column enzyme reactor via a covalent bond with glutaraldehyde and aminopropyl controlled-pore glass. It has also been used in a study to investigate the use of tyrosine, histidine, and cysteine as ligands for Mn(III) in sweet potato phosphatase.

Biochem/physiol Actions

Acid phosphatases (APase) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimum pH of 4 to 7 by the following reaction: APaseR-PO₄ + H₂O --------------- R-OH + HOPO₃ ²⁺Their function in the production, transport, and recycling of phosphate is critical for the metabolic and energy transduction processes of the cell. As a group, Apases may be as important as kinases in regulatory processes.

Physical form

Suspension in 1.8 M (NH₄)₂SO₄, 10 mM MgCl₂, pH 5.3

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)