Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

P1411

Sigma-Aldrich

Polymyxin B-Agarose

aqueous glycerol suspension

Synonym(s):

PMB

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

MDL number:
MFCD00165491
UNSPSC Code:
41106500
NACRES:
NA.56
Pricing and availability is not currently available.

biological source

synthetic (organic)

Quality Level

200

form

aqueous glycerol suspension

extent of labeling

~1 mg per mL

technique(s)

affinity chromatography: suitable

matrix

Cross-linked 4% beaded agarose

matrix activation

cyanogen bromide

matrix attachment

amino

matrix spacer

1 atom

capacity

200-500 μg/mL binding capacity (lipopolysaccharide)(from Escherichia coli serotype 0128:B12)

suitability

suitable for chromatography

storage temp.

2-8°C

Application

Polymyxin B-agarose is used in protein chromatography, affinity chromatography and specialty resins. Polymyxin B-agarose has been used to study periodontitis and endodontic infections as well as obesity and type 2 diabetes.
Useful for removal of endotoxins from solutions

Physical form

Suspension in 50% glycerol containing 0.02% sodium azide

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000375992
0000364940
0000348645
0000375992
0000364940

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Martín López et al.
Journal of immunology (Baltimore, Md. : 1950), 170(5), 2409-2416 (2003-02-21)
Macrophages infected with Mycobacterium tuberculosis undergo increased rates of apoptosis. Important objectives are to define the microbial factors that cause apoptosis, the mechanisms involved and the impact on infection. The 19-kDa M. tuberculosis glycolipoprotein (p19) is both cell wall-associated and
A C Issekutz
Journal of immunological methods, 61(3), 275-281 (1983-07-29)
Endotoxins liberated by gram-negative bacteria are frequent contaminants of aqueous and physiological solutions. Because of their potent biological effects in vivo and in vitro, it is often necessary to eliminate even minute quantities of endotoxin from such solutions. A process
Dina B AbuSamra et al.
Cells, 10(2) (2021-02-13)
Chemokines are an extended group of chemoattractant cytokines responsible for the recruitment of leukocytes into tissues. Among them, interferon-γ-inducible protein 10 (CXCL10) is abundantly expressed following inflammatory stimuli and participates in the trafficking of monocytes and activated T cells into
P J Jagielo et al.
The American journal of physiology, 270(6 Pt 1), L1052-L1059 (1996-06-01)
To investigate the role of endotoxin in grain dust-induced airway inflammation, we reduced the endotoxin activity from extracts of corn dust (CDE), using three distinct methods, and determined the effect of endotoxin activity on the in vitro and in vivo
Sesselja Omarsdottir et al.
International immunopharmacology, 6(11), 1642-1650 (2006-09-19)
Many naturally occurring polysaccharides from fungi and lichens have been found to have immunomodulating activity. However, the majority of these studies have focused on their effects on the innate arm of the immune system. Although dendritic cells (DCs) belong to
View All Related Papers

Questions

1–2 of 2 Questions  

  1. What polypropylene column should the we use according to the protocol to wash the resin prior to use?

    1 answer

    1. For washing the resin prior to use, the customer can use the following options:

      1. C2603 Mini-Columns: These have a capacity of 0.5 mL and an overall height of 1.9 inches.

      2. C2728 Mini-Columns: These have a capacity of 2.4 mL and an overall height of 1.5 inches.

      The washing procedure using Polymyxin B Agarose (Product No. P1411) can be carried out in a column or in a batch method. For a column, the customer can use disposable mini columns, such as Product Nos. C2603 (0.5 ml) and C2728 (5.0 ml). If a larger column is needed, the customer can use a polypropylene syringe barrel or use the batch method.

      In the batch method, the solution is incubated with the polymyxin B agarose for a minimum of 30 minutes with rotation in either a 15 ml or 50 ml polypropylene tube. The tube is then centrifuged, and the supernatant contains the protein of interest with decreased endotoxin that has been bound to the beads and pelleted in the tube.

      Helpful?

  2. What methods can be used to remove endotoxin from a product?

    1 answer

    1. The product is not tested for endotoxin; however, P1411 may be used to strip endotoxin. The P1411 datasheet includes a suggested protocol for this process. The protocol involves washing the resin, equilibrating the column, dissolving the sample in buffer, eluting with endotoxin-free buffer, and collecting the fractions. The collected fractions containing the protein should then be lyophilized. The polymyxin B agarose can be regenerated at least 10 times, and the resin can be stored in 25% ethanol at 4°C

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service