The volume may be determined using the lot specific Enzymatic Activity reported in the Certificate of Analysis. For example, if a specific lot reports an Enzymatic Activity of 7.17 U/mL, the 1 unit pack size will have a volume of roughly 139 uL and the 2 unit pack size would have a volume of 278 uL. Please see the link below to review a sample or lot specific Certificate of Analysis:
https://www.sigmaaldrich.com/US/en/product/sigma/n7885#product-documentation
N7885
Neuraminidase from Vibrio cholerae
Type III, buffered aqueous solution, 0.2 μm filtered, 1-5 units/mg protein (Lowry, using NAN-lactose)
Synonym(s):
Acyl-neuraminyl Hydrolase, Receptor-destroying enzyme, Sialidase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-624-6
MDL number:
EC Number:
Specific activity:
1-5 units/mg protein (Lowry, using NAN-lactose)
Pricing and availability is not currently available.
Quality Segment
type
Type III
form
buffered aqueous solution
specific activity
1-5 units/mg protein (Lowry, using NAN-lactose)
mol wt
83 kDa
application(s)
diagnostic assay manufacturing
foreign activity
Protease and NAN-aldolase, present
storage temp.
2-8°C
General description
Neuraminidase enzymes are glycoside hydrolase enzymes that catalyze hydrolysis of terminal sialic acid residues. The most well-known are the viral nearamidases, which promote influenza virus release.
Application
Neuraminidase from Vibrio cholerae has been used in a study to assess its role in the binding and uptake of cholera toxin by susceptible cells. It has also been used in a study to investigate the preparation of a sodium substrate and its use in a fluorometric assay of neuraminidase.
Neurminidase is used as a cell-surface probe for glycoconjugate distribution and in substrate specificity studies.
Biochem/physiol Actions
Physical form
Aqueous solution, pH 5.5, containing 0.15 M NaCl and 4 mM CaCl2.
Preparation Note
Chromatographically purified.
Analysis Note
Preservative free.
Other Notes
One unit will liberate 1.0 μmole of N-acetylneuraminic acid per min at pH 5.0 at 37 °C using NAN-lactose or bovine submaxillary mucin, unless otherwise specified. Prices based on units using NAN-lactose as substrate.
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This Item | |||
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| description Type III, buffered aqueous solution, 0.2 μm filtered, 1-5 units/mg protein (Lowry, using NAN-lactose) | description Type II, buffered aqueous solution, 8-24 units/mg protein (Lowry, using NAN-lactose) | description ≥1.5 U/mL, specific activity ≥ 1.5U/mg protein | description Type VIII, lyophilized powder, ≥10 units/mg protein (using 4MU-NANA), ≥3.5 units/mg protein (mucin) |
| specific activity 1-5 units/mg protein (Lowry, using NAN-lactose) | specific activity 8-24 units/mg protein (Lowry, using NAN-lactose) | specific activity ≥1.5 U/mg protein | specific activity ≥10 units/mg protein (using 4MU-NANA), ≥3.5 units/mg protein (mucin) |
| form buffered aqueous solution | form buffered aqueous solution | form liquid | form lyophilized powder |
| application(s) diagnostic assay manufacturing | application(s) - | application(s) - | application(s) - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| mol wt 83 kDa | mol wt - | mol wt - | mol wt - |
| Quality Level 300 | Quality Level 200 | Quality Level - | Quality Level 200 |
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Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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Dear Customer service I have a question regarding the product (N7885 Neuraminidase from Vibrio cholerae). In how much volume does the 1 Unit or 2 Unit package arrive? Could you provide a volume (or mg if it's powder)? Thanks, Ben
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