N6875

2-Naphthyl acetate

• Synonym(s): β-Naphthyl acetate
• Linear Formula: CH₃CO₂C₁₀H₇
• CAS Number: 1523-11-1
• Molecular Weight: 186.21
• UNSPSC Code: 12352204
• NACRES: NA.32
• PubChem Substance ID: 24897794
• EC Number: 216-194-7
• Beilstein/REAXYS Number: 1868291
• MDL number: MFCD00004060

Properties

• form: powder
• Quality Segment: 100
• impurities: <0.2% Free naphthol
• mp: 67-70 °C (lit.)
• solubility: ethanol: 50 mg/mL
• storage temp.: −20°C
• SMILES string: CC(=O)Oc1ccc2ccccc2c1
• InChI: 1S/C12H10O2/c1-9(13)14-12-7-6-10-4-2-3-5-11(10)8-12/h2-8H,1H3
• InChI key: RJNPPEUAJCEUPV-UHFFFAOYSA-N

Description

Application

2-Naphthyl acetate has been used as a substrate:

• in substrate sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to examine the composition of lipases in larvae and adult specimens
• in the preparation of zymograms to identify proteins with lipase activity
• to measure non-specific esterase activity and β-esterase enzyme activity

Biochem/physiol Actions

2-Naphthyl acetate/β-naphthyl acetate (BNA) is an ester substrate. It can be used in staining gels to detect esterase activity. BNA dissolved in acetone and ethanol produces a dark and very light/no background respectively. Hence destaining is not necessary if ethanol is used to dissolve BNA for esterase staining of gels.

Safety Information

• pictograms: GHS05
• signalword: Danger
• hcodes: H318
• pcodes: P280 - P305 + P351 + P338
• Hazard Classifications: Eye Dam. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves