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NZCYM Broth

Name: NZCYM Broth
Brand: SIGMA

Powder microbial growth medium

Synonym(s):

Broth medium

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About This Item

NACRES:

NA.85

UNSPSC Code:

41106200

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Properties

grade

Molecular Biology

Quality Segment

200

sterility

non-sterile

form

powder

composition

Casamino Acids, 1 g/L , MgSO₄, 0.98 g/L , NaCL, 5 g/L , Tryptone, 10 g/L , Yeast extract, 5 g/L

technique(s)

microbiological culture: suitable

pH

6.8-7.2(2.2% solution)

application(s)

food and beverages
microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli, nonselective for coliforms

Description

General description

NZCYM is a microbial growth medium used for the cultivation of E. coli and lambda bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.

Application

NZCYM Broth has been used for non-selective cultivation of lambda, MS2, T7, CLP-P3, Phi29, and Goe1 bacteriophages, and E. coli strains for cloning, DNA plasmid production, and production of recombinant proteins. It is also suitable for selective cultivation when appropriate antibiotics are added.

Features and Benefits

NZCYM powder provides:

Standard formulation
Convenient package sizes
A budget-friendly alternative to liquid

Preparation Note

Stir to suspend 22 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

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This Item	L7533	L3022	L3522
Sigma-Aldrich N3643 NZCYM Broth	Sigma-Aldrich L7533 LB Broth with agar (Lennox)	Sigma-Aldrich L3022 LB Broth (Lennox)	Sigma-Aldrich L3522 LB Broth (Miller)
form powder	form powder	form powder	form powder
suitability nonselective for Escherichia coli, nonselective for coliforms	suitability nonselective for Escherichia coli, nonselective for coliforms	suitability nonselective for Escherichia coli, nonselective for coliforms	suitability nonselective for Escherichia coli, nonselective for coliforms
technique(s) microbiological culture: suitable	technique(s) microbiological culture: suitable	technique(s) microbiological culture: suitable	technique(s) -
sterility non-sterile	sterility non-sterile	sterility non-sterile	sterility non-sterile
composition Casamino Acids, 1 g/L , NaCL, 5 g/L , Yeast extract, 5 g/L , MgSO₄, 0.98 g/L , Tryptone, 10 g/L	composition Agar, 15 g/L , NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L	composition NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L	composition NaCl, 10 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L
application(s) food and beverages microbiology	application(s) agriculture food and beverages microbiology	application(s) food and beverages microbiology	application(s) food and beverages

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Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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