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M8662

Mineral oil

PCR Reagent

Synonym(s):

Mineral oil for PCR

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About This Item

CAS Number:
UNSPSC Code:
12181504
NACRES:
NA.52
EC Number:
232-455-8
MDL number:
Technique(s):
PCR: suitable
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Quality Segment

form

liquid

feature

PCR Additives

packaging

vial of 1.5 mL (Total volume 7.5 mL (5 vials))

technique(s)

PCR: suitable

color

colorless

refractive index

n20/D 1.467 (lit.)

density

0.84 g/mL at 25 °C (lit.)

foreign activity

DNase, RNase, protease, none detected

storage temp.

room temp

InChI key

AEOVEGJBKQQFOP-DDVLFWKVSA-L

General description

Mineral oil is used as a reaction mix overlay in polymerase chain reactions (PCR) to prevent reaction solutions from evaporating during the thermal cycling in PCR instruments without heated lids.

Application

Mineral oil has been used:
  • for routine PCR amplifications
  • to improve the heat conductivity between the device and the cycler during reverse transcription-polymerase chain reaction (RT–PCR) amplification[1]
  • to prevent evaporation during single-cell MATQ-sequencing performed on Bravo automated liquid handling platform[2][3]

Features and Benefits

  • Provided in a convenient 5 × 1.5 mL (1 vial) pack size
  • Tested for the absence of DNase, RNase, and protease.

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M3516M5904M5310
Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

300

technique(s)

PCR: suitable

technique(s)

PCR: suitable

technique(s)

PCR: suitable

technique(s)

cell culture | embryo: suitable

form

liquid

form

light oil

form

light oil

form

light oil

density

0.84 g/mL at 25 °C (lit.)

density

0.84 g/mL at 25 °C (lit.)

density

0.84 g/mL at 25 °C (lit.)

density

0.82-0.88 g/mL at 25 °C

refractive index

n20/D 1.467 (lit.)

refractive index

n20/D 1.467 (lit.)

refractive index

n20/D 1.467 (lit.)

refractive index

n20/D 1.467 (lit.)

feature

PCR Additives

feature

-

feature

-

feature

-


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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

No data available

flash_point_c

No data available

ppe

Eyeshields, Gloves



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