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M4526

Minimum Essential Medium Eagle

Alpha Modification, with sodium bicarbonate and Earl's salts, without ʟ-glutamine, ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture

Minimum Essential Medium Eagle

Synonym(s):

αMEM, EMEM, MEM

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71
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Product Name

Minimum Essential Medium Eagle, Alpha Modification, with sodium bicarbonate, without L-glutamine, ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture

Quality Segment

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

Earle’s salts (5% CO2): yes
sodium pyruvate: yes
glucose: yes
L-glutamine: no
HEPES: no
Hanks’ salts (2% CO2): no
phenol red: yes
NaHCO3: yes
stable glutamine: no

shipped in

ambient

storage temp.

2-8°C

General description

Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has enhanced the uses of this medium. The α modification of MEM with Earle′s balanced salts also known as αMEM, contains non-essential amino acids, sodium pyruvate, and additional vitamins.
This is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins.

Application

Minimum Essential Medium Eagle has been used to maintain:
  • immature cumulus-oocyte complexes from mice[1]
  • human mesenchymal stem cells (MSC)[2]
  • mouse pre-osteoblastic cell line[3]

Preparation Note

Supplement with 0.292 g/L L-glutamine.

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This Item
M8042M5650M0644
Minimum Essential Medium Eagle Alpha Modification, with sodium bicarbonate, without L-glutamine, ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

M4526

Minimum Essential Medium Eagle

Minimum Essential Medium Eagle Alpha Modification, with ribonucleosides, deoxyribonucleosides and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

M8042

Minimum Essential Medium Eagle

Minimum Essential Medium Eagle With Earle′s salts, non-essential amino acids and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

M5650

Minimum Essential Medium Eagle

Sigma-Aldrich

Sigma-Aldrich

M0644

Minimum Essential Medium Eagle

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

liquid

form

liquid

form

liquid

form

powder

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

components

Earle’s salts (5% CO2): yes
sodium pyruvate: yes
glucose: yes
L-glutamine: no
HEPES: no
Hanks’ salts (2% CO2): no
phenol red: yes
NaHCO3: yes
stable glutamine: no

components

Hanks’ salts (2% CO2): no
glucose: yes
sodium pyruvate: yes
phenol red: yes
HEPES: no
L-glutamine: no
stable glutamine: no
Earle’s salts (5% CO2): yes
NaHCO3: yes

components

phenol red: yes
NaHCO3: yes
L-glutamine: no
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes

components

NaHCO3: no
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
RNBM0562
RNBL7706
RNBL2659
RNBL5431
RNBL0707

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Philipp von Eisenhart-Rothe et al.
Investigative ophthalmology & visual science, 59(12), 5082-5097 (2018-10-30)
Vision loss caused by photoreceptor death represents one of the first symptoms in neuronal ceroid lipofuscinosis, a condition characterized by accumulation of intracellular waste. Cln6nclf mice have a naturally occurring mutation in ceroid-lipofuscinosis neuronal (CLN) protein 6 and are a
Zhourui Ma et al.
Journal of molecular histology, 51(3), 241-250 (2020-05-14)
Using a large-scale quantitative mesenchymal stem cells (MSCs) membrane proteomics analysis, we identified a large group of ciliary proteins in the MSCs membrane fraction, which prompted us to examine the cilia, intricate organelles that were originally discovered approximately 100 years ago.
Michelle L Churchman et al.
Cancer cell, 33(5), 937-948 (2018-04-24)
Somatic genetic alterations of IKZF1, which encodes the lymphoid transcription factor IKAROS, are common in high-risk B-progenitor acute lymphoblastic leukemia (ALL) and are associated with poor prognosis. Such alterations result in the acquisition of stem cell-like features, overexpression of adhesion
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Global Trade Item Number

SKUGTIN
M4526-500ML04061834059004
M4526-24X500ML04061834058991
M4526-6X500ML04061834059011
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