L9017

Lectin from Phaseolus vulgaris (red kidney bean)

BioXtra, Phytohemagglutinin PHA-P, purified by affinity chromatography, lyophilized powder

• Synonym(s): Phaseolus vulgaris agglutinin, PHA
• UNSPSC Code: 12352202
• EC Number: 232-718-7
• NACRES: NA.32
• Biological source: Phaseolus vulgaris

Properties

• biological source: Phaseolus vulgaris
• Quality Segment: 300
• product line: BioXtra
• form: lyophilized powder
• potency: ≤17 μg per mL agglutination activity
• purified by: affinity chromatography
• impurities: salt, essentially free
• storage temp.: 2-8°C

Description

General description

Lectins are carbohydrate-binding proteins, that contains four subunits with a molecular weight of 125 kDa. Leucoagglutinin PHA-L is a plant lectin, which is transported by the neurons in the central nervous system.

Application

Lectin from Phaseolus vulgaris (red kidney bean) has been used:

• for phytohemagglutinin (PHA) swelling assay
• for lymphocyte activation assay
• to evaluate cell-mediated immune response

Biochem/physiol Actions

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Lectin agglutinates cells and precipitates polysaccharides and glycoproteins. It has anti-cancer, anti-human immunodeficiency virus (anti-HIV) and anti-microbial infection properties. Lectin is associated with toxic allergens and hemagglutinins. Lectin inhibits mucosal atrophy, lowers type 2 diabetes and obesity, enhances nutrient absorption and regulates drug targeting.

Analysis Note

Agglutination activity is expressed in mg/mL and is determined from serial dilutions of a 1 mg/mL solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8 after 1 hr incubation at 25 °C. Mitogenic activity is determined by BrdU incorporation.

Mitogenic activity at ≤10 μg per mL.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable