L8754

Lectin from Phaseolus vulgaris (red kidney bean)

Phytohemagglutinin PHA-P, lyophilized powder

• Synonym(s): Phaseolus vulgaris agglutinin, PHA
• UNSPSC Code: 12352202
• EC Number: 232-718-7
• NACRES: NA.32
• Form: lyophilized powder
• Biological source: Phaseolus vulgaris

Properties

• biological source: Phaseolus vulgaris
• Quality Segment: 300
• form: lyophilized powder
• potency: ≤62.5 μg per mL agglutination activity, ≤10 μg/mL mitogenic activity
• impurities: salt, free
• storage temp.: 2-8°C

Description

General description

PHAs are carbohydrate-binding proteins. There are several types of PHAs identified, predominantly in leguminous plants.

Phytohaemagglutinin (PHA, or phytohemagglutinin) is a lectin found in plants (esp. in legumes). It is obtained from Phaseolus vulgaris (red kidney beans) and acts as a mitogen in human leucocyte culture.

Application

It has also been used as an antigen to measure in vivo T-cell-mediated immune responsiveness.

Lectin from Phaseolus vulgaris (red kidney bean) has been used in phytohemagglutinin (PHA) assay and during assessment of cellular and humoral immunity.

Biochem/physiol Actions

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Analysis Note

Agglutination activity is expressed in mg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C. Mitogenic activity is determined by BrdU incorporation in HeLa cell cultures.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable