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L7920

β-Lactamase Blend

recombinant, expressed in E. coli

Synonym(s):

Carbapenemase, Cephalosprinase

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About This Item

EC Number:
3.5.2.6 (BRENDA, IUBMB)
NACRES:
NA.54
UNSPSC Code:
12352204
Recombinant:
expressed in E. coli
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recombinant

expressed in E. coli

Quality Segment

200

sterility

γ-irradiated

form

lyophilized powder

storage temp.

2-8°C

General description

No detectable growth in tryptone soy broth at 30-35 °C for 14 days

Biochem/physiol Actions

β-Lactamases exhibit high carbapenemase activity. β-lactamases can destroy the β-lactam ring. True carbapenemases has the ability to hydrolyse several β-lactam ring containing carbapenems like meropenem, doripenem, imipenem and ertapenem.

Physical form

Lyophilized powder containing sodium chloride, potassium phosphate, dipotassium phosphate and sorbitol.

Other Notes

600-1500 IU beta-lactamase I per vial
60-150 IU beta-lactamase II per vial
One unit will hydrolyze 1.0 μmole substrate per min (βI: benzylpenicillin; βII: cephalosporin C) in phosphate buffer, pH 7.0 at 25°C.

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β-Lactamase Blend recombinant, expressed in E. coli

Sigma-Aldrich

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β-Lactamase from Pseudomonas fluorescens lyophilized powder, recombinant, expressed in E. coli

Sigma-Aldrich

L3045

β-Lactamase from Pseudomonas fluorescens

β-Lactamase from Enterobacter cloacae Type IV, lyophilized powder, 0.2-0.6 units/mg protein (using benzylpenicillin)

Sigma-Aldrich

P4524

β-Lactamase from Enterobacter cloacae

β-Lactamase recombinant, expressed in E. coli

Sigma-Aldrich

L6170

β-Lactamase

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

powder

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

-

recombinant

expressed in E. coli

sterility

γ-irradiated

sterility

-

sterility

-

sterility

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

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Questions

1–2 of 2 Questions  

  1. Dear Mr/Ms, I ordered β-Lactamase Blend L7920 and I am interested in knowing the concentration in micrograms per mL. (Micrograms per mL=IU/mL/min×Conversion Factor). Can you please tell me what is the conversion factor? Regards, Glorjen

    1 answer

    1. This product is provided in lyophilized solid form with the unit activity reported in lot specific Certificate of Analysis.

      Using a representative lot, SLCQ2635 as an example, one vial contains 1289 units. The activity can be converted to mass in milligrams using the batch specific activity value of 124 units/mg.

      Thus, a vial from batch (SLCQ2635) contains 10.39 mg of enzyme; 1289 units x (1 mg / 124 unit) = 10.39 mg.

      The volume in mL would be dependent on the volume of solvent added. For example, adding 1 mL of solvent to a vial from this batch, would result in a concentration of 10.39 mg/mL.

      Helpful?

  2. HOW TO DISSOLVE THE VIAL

    1 answer

    1. This product has been sterilized by gamma-irradiaton and can be reconstituted with in 10 mL of deionized purified water, then filter sterilzed through a 0.2 micron low protein binding membrane. If being used for the preparation of agar plates, it should be added to the medium at a temperature of less than 55 deg C. After reconstitution, the solution is stable for 2 months at 2-8 deg C."

      Helpful?

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