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Lipopolysaccharides from Serratia marcescens

Name: Lipopolysaccharides from <I>Serratia marcescens</I>
Brand: SIGMA

purified by phenol extraction

Synonym(s):

LPS

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About This Item

UNSPSC Code:

12352201

NACRES:

NA.25

MDL number:

MFCD00131520

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Properties

biological source

Serratia marcescens

Quality Segment

200

form

lyophilized powder

purified by

phenol extraction

impurities

<3% Protein (Lowry)

color

white to yellow cast

solubility

water: 4.90-5.10, faintly hazy to hazy, colorless to faint yellow or tan

shipped in

ambient

storage temp.

2-8°C

Description

General description

This product is phenol extracted from Serratia marcescens. The source strain is ATCC 21639.

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 ^oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

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This Item	L6386	L4268	L9143
Sigma-Aldrich L6136 Lipopolysaccharides from Serratia marcescens	Sigma-Aldrich L6386 Lipopolysaccharides from Salmonella typhosa	Sigma-Aldrich L4268 Lipopolysaccharides from Klebsiella pneumoniae	Sigma-Aldrich L9143 Lipopolysaccharides from Pseudomonas aeruginosa 10
biological source Serratia marcescens	biological source bacterial (Salmonella typhosa)	biological source bacterial (Klebsiella pneumoniae)	biological source Pseudomonas aeruginosa (10)
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
solubility water: 4.90-5.10, faintly hazy to hazy, colorless to faint yellow or tan	solubility water: 4.90-5.10 mg/mL, faintly hazy to hazy, colorless to faintly yellow	solubility water: 4.90-5.10 mg/mL, slightly hazy to turbid, colorless to faintly yellow	solubility water: 4.90-5.10 mg/mL, faintly hazy to hazy, colorless to faintly yellow
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
color white to yellow cast	color white to yellow cast	color white to faint yellow	color white to faint yellow

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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