I7908

IL-2 human

≥98% (SDS-PAGE and HPLC), recombinant, expressed in Pichia pastoris, lyophiilized powder, suitable for cell culture

• Synonym(s): IL-2, Interleukin-2, T-cell growth factor
• UNSPSC Code: 12352202
• NACRES: NA.75
• MDL number: MFCD00165721
• Biological source: human
• Recombinant: expressed in Pichia pastoris
• Assay: ≥98% (SDS-PAGE and HPLC)
• Form: lyophilized powder
• Mol wt: predicted mol wt ~15 kDa
• Impurities: endotoxin, tested

Properties

• Product Name: Interleukin-2 human, recombinant, expressed in Pichia pastoris, suitable for cell culture
• biological source: human
• Quality Segment: 200
• recombinant: expressed in Pichia pastoris
• assay: ≥98% (SDS-PAGE and HPLC)
• form: lyophilized powder
• mol wt: predicted mol wt ~15 kDa
• packaging: pkg of 10KU
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• UniProt accession no.: P60568
• shipped in: dry ice
• storage temp.: −20°C

Description

Application

Interleukin-2 human has been used to assess changes in tumor necrosis factor receptor 1 (TNFR1). It has also been used in TNFR2 expression on Tregs.

Biochem/physiol Actions

Interleukin 2 (IL2) is a pro-inflammatory cytokine. It consists of three subunits, in which the β subunit is important for receptor mediated signaling. IL2 enhances the development of T regulatory cells and plays a crucial role in antitumor immunity.

Interleukin-2 (IL-2) is an immunomodulatory factor produced by certain subsets of T lymphocytes. This lympho­kine promotes long term growth of acti­vated T cells and related cell types. IL-2 plays a role in the activation and proliferation of NK cells, induces γ-inter­feron and B cell growth factor secretion, and modulates the expression of the IL-2 receptor.

Physical form

Lyophilized from a 0.2 μm filtered solution in 50 mM sodium phosphate buffer, pH 7.0, containing 0.1% human serum albumin per 0.1 mL of cytokine.

Analysis Note

The biological activity is tested in a cell proliferation assay using an IL-2 dependent murine cytotoxic T cell line, CTLL-2.

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)