I5635

Monoclonal Anti-Human IgG2 antibody produced in mouse

clone HP-6014, ascites fluid

• Synonym(s): Monoclonal Anti-Human IgG2
• NACRES: NA.46
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: HP-6014, monoclonal
• Application: ELISA (i)
• Citations: 14

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: secondary antibodies
• clone: HP-6014, monoclonal
• contains: 15 mM sodium azide
• technique(s): indirect ELISA: 1:5,000
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Monoclonal Anti-Human IgG2 (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Human IgG consists of four subclasses.

Application

Monoclonal Anti-Human IgG2 antibody has been used in in enzyme linked immunosorbent assay (ELISA) and in western blotting.
It may also be used:

• in imprint immunofixation
• in immunofluorometric assay
• in hemagglutination
• in hemagglutination inhibition
• in particle counting immunoassay
• in detection of cytoplasmic IgG

Biochem/physiol Actions

Anti-polysaccharide responses are mainly of the IgG2 sub-class while protein antigens give rise to IgG1 and IgG3 antibodies. Lipopolysaccharides stimulate an IgG2 response in peripheral blood leukocytes (PBL′s).

The antibody is specific for human IgG2 and is non-reactive with other IgG subclasses. This clone has been singled out as the most widely applicable IgG2 specific monoclonal antibody by the IUIS/WHO study.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable