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For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
I5513
Imidazole
Molecular Biology, ≥99% (titration)
Synonym(s):
3-Azapyrrole, 1,3-Diaza-2,4-cyclopentadiene, Glyoxaline
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| Pack Size | SKU | Availability | Price |
|---|
About This Item
grade
Molecular Biology
Quality Segment
vapor pressure
<1 mmHg ( 20 °C)
assay
≥99% (titration)
form
crystalline powder
storage condition
dry at room temperature
technique(s)
electrophoretic mobility shift assay: suitable, molecular cloning: suitable, protein purification: suitable
color
white
pH
6.2-7.8 (25 °C)
pKa (25 °C)
6.95
bp
256 °C (lit.)
mp
88-91 °C (lit.)
solubility
water: 500 mg/mL, clear, colorless to very faintly yellow
density
1.004 g/cm3 at 20—25 °C, 1.006 g/cm3 at 20—25 °C
suitability
suitable for molecular biology
application(s)
diagnostic assay manufacturing
genomic analysis
life science and biopharma
microbiology
foreign activity
DNase, RNase and protease, none detected
storage temp.
room temp
SMILES string
c1c[nH]cn1
InChI
1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5)
InChI key
RAXXELZNTBOGNW-UHFFFAOYSA-N
General description
Furthermore, imidazole is indispensable for enhancing protein purity. It is widely used in preparing buffer solutions optimized for downstream purification columns. Imidazole solutions are also effective elution and washing agents throughout protein purification processes. Additionally, imidazole can be used for assays of horseradish peroxidase (HRP) and reverse staining of SDS-PAGE gels for protein detection. Imidazole is a versatile and valuable reagent for a wide range of biological applications. It is an essential component of many buffers and is also used in protein purification and detection protocols.
Application
- in the lysis, wash and elution buffer for the purification of histidine tagged Sonic Hedgehog(shh-N) protein
- in elution buffer in stepwise gradient for the purification of histidine tagged aldo keto reductases using nickel affinity chromatography
- as a component of homogenization buffer for the purification of phagosomal compartments from dendritic cells
- as a binding buffer, wash buffer, and elution buffer in a study involving protein expression and purification of bioactive growth factors for use in cell culture and cellular agriculture
Biochem/physiol Actions
Features and Benefits
- Suitable for Molecular Biology and Biochemical Research
- Can be used as a Buffer component, for Protein separation
- Free from DNase, NICKase, RNase, and Protease
- Effective Buffering from pH 6.2 to 7.8 (25 °C) with a pKa of 6.9 (25 °C)
Other Notes
1 of 1
This Item | |||
|---|---|---|---|
| grade Molecular Biology | grade - | grade ACS reagent | grade - |
| assay ≥99% (titration) | assay 99% | assay ≥99% (titration) | assay ≥99.5% (GC) |
| technique(s) electrophoretic mobility shift assay: suitable, protein purification: suitable, molecular cloning: suitable | technique(s) - | technique(s) - | technique(s) - |
| Quality Level 200 | Quality Level 100 | Quality Level 300 | Quality Level 200 |
| form crystalline powder | form powder | form - | form flakes, powder or crystals |
| application(s) diagnostic assay manufacturing | application(s) - | application(s) - | application(s) - |
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signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Eye Dam. 1 - Repr. 1B - Skin Corr. 1C
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 2
flash_point_f
293.0 °F - closed cup
flash_point_c
145 °C - closed cup
ppe
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| I5513-100G | 04061835545766 |
| I5513-25G | 04061835545773 |
| I5513-5G | 04061835545780 |
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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