I4269

IL-4 human

≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): IL-4, Interleukin-4 human, hIL-4
• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD00151705
• Biological source: human
• Recombinant: expressed in E. coli
• Assay: ≥98% (SDS-PAGE)
• Form: lyophilized powder
• Mol wt: 14.9 kDa (containing 129 amino acid residues)
• Impurities: ≤1.000 EU/μg

Properties

• Product Name: Interleukin-4 human, IL-4, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• biological source: human
• Quality Level: 200
• recombinant: expressed in E. coli
• assay: ≥98% (SDS-PAGE)
• form: lyophilized powder
• potency: ≤0.400 ng/mL EC₅₀
• quality: endotoxin tested
• mol wt: 14.9 kDa (containing 129 amino acid residues)
• packaging: pkg of 5 and 50 μg
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1.000 EU/μg
• color: white
• UniProt accession no.: P05112
• storage temp.: −20°C
• Gene Information: human ... IL4(3565) ; mouse ... IL4(3565)

Description

General description

Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including differentiation of naive T cells into the TH2 phenotype, promoting B cell proliferation, antibody isotype switching, and expression of other TH2 cytokines including IL-5 and IL-9.^1,2 IL-4 plays a critical role in the development of allergic inflammation and asthma.³

IL-4 binds to two distinct receptors; the type I receptor, a heterodimer consisting of the IL-4Rα chain and the common gamma chain, γc, and the type II receptor, a heterodimer of IL-4Rα and IL-13Rα1.⁴

Naturally occurring IL-4 has a molecular mass of 12-20 kDa. Recombinant human IL-4 is a 14.9 kDa protein containing 129 amino acid residues. Human and mouse IL-4 share a 50% amino acid sequence homology, but their biological actions are species-specific.⁵

Application

Lymphokine with profound effects on the growth and differentiation of immunologically competent cells, inhibits VEGF-induced and βFGF-induced angiogenesis.

Physical form

Lyophilized from a sterile filtered solution.

Analysis Note

The biological activity is measured by the dose-dependent stimulation of the proliferation of human TF-1 cells.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable