Insulin in solution is reasonably stable. Sterile solutions can be stored at 2–8 °C for up to 12 months. Should freezing be preferred, solutions may be stored frozen at -20°C in single-use aliquots. Freeze-thaw cycles should be avoided.
I3536
Insulin human
recombinant, expressed in yeast, γ-irradiated, suitable for cell culture
Synonym(s):
Insulin human
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About This Item
Empirical Formula (Hill Notation):
C257H383N65O77S6
CAS Number:
Molecular Weight:
5807.57
UNSPSC Code:
12352209
NACRES:
NA.77
MDL number:
Biological source:
Saccharomyces cerevisiae
Form:
lyophilized powder
Pricing and availability is not currently available.
biological source
Saccharomyces cerevisiae
Quality Level
recombinant
expressed in yeast
sterility
γ-irradiated
form
lyophilized powder
potency
≥25 USP units per mg
technique(s)
cell culture | mammalian: suitable
solubility
0.01 M HCl: 20 mg/mL, clear, colorless to faintly yellow
UniProt accession no.
shipped in
ambient
storage temp.
−20°C
InChI key
PBGKTOXHQIOBKM-FHFVDXKLSA-N
Gene Information
human ... INS(3630)
General description
The INS gene encodes for preproinsulin, which is enzymatically converted into insulin.[1] Insulin is produced in the insulin-producing pancreatic β cells.[2] Preproinsulin is converted to proinsulin in ER and proinsulin is then proteolytically processed to form insulin in newly-forming insulin secretory granules.[3] Insulin production is tightly regulated by specific DNA elements present within ∼400 bp in the proximal region of the INS promoter.[4]
Application
Biochem/physiol Actions
Insulin is responsible for two types of actions- excitatory and inhibitory. In its excitatory role, it increases the uptake of glucose and lipid synthesis, and in its inhibitory role it inhibits glycogenolysis, gluconeogenesis, lipolysis, proteolysis and ketogenesis.[8] Aberrant insulin secretion leads to various disorders such as diabetes, hyperglycemia or hypoglycaemia.[2] Type I diabetes is a result of autoimmune destruction of β cells of pancreas, which leads to depletion of insulin.[9] Mutant INS-gene Induced Diabetes of Youth (MIDY) syndrome is an autosomal dominant disorder caused by missense mutations, which lead to aberrant proinsulin folding.[3] Impaired glucose tolerance (IGT) or non-insulin-dependent diabetes mellitus (NIDDM) is caused by resistance to insulin-stimulated glucose uptake.[10]
Regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat.
Two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800 Da. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond. Insulin regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat.
Other Notes
InChI: 1S/C257H383N65O77S6/c1-29-131(23)205(313-193(339)104-259)252(393)317-204(130(21)22)248(389)288-159(75-82-200(349)350)217(358)282-156(71-78-189(263)335)221(362)308-183-116-403-404-117-184-243(384)305-178(111-324)240(381)294-162(88-123(7)8)225(366)295-168(95-140-53-61-146(329)62-54-140)228(369)283-154(69-76-187(261)333)218(359)290-161(87-122(5)6)223(364)285-158(74-81-199(347)348)220(361)302-174(101-190(264)336)235(376)298-170(97-142-57-65-148(331)66-58-142)231(372)309-182(242(383)304-176(255(396)397)103-192(266)338)115-402-401-114-181(214(355)273-107-194(340)278-153(72-79-197(343)344)216(357)281-151(51-42-84-271-257(267)268)212(353)272-108-195(341)279-166(93-138-46-36-32-37-47-138)227(368)297-167(94-139-48-38-33-39-49-139)230(371)299-171(98-143-59-67-149(332)68-60-143)238(379)320-208(135(27)327)254(395)322-85-43-52-186(322)246(387)286-152(50-40-41-83-258)222(363)321-209(136(28)328)256(398)399)311-250(391)203(129(19)20)316-236(377)164(90-125(11)12)292-229(370)169(96-141-55-63-147(330)64-56-141)296-224(365)160(86-121(3)4)289-210(351)133(25)277-215(356)157(73-80-198(345)346)287-247(388)202(128(17)18)315-237(378)165(91-126(13)14)293-233(374)173(100-145-106-270-120-276-145)301-239(380)177(110-323)280-196(342)109-274-213(354)180(113-400-405-118-185(310-244(183)385)245(386)319-207(134(26)326)253(394)306-179(112-325)241(382)318-206(132(24)30-2)251(392)312-184)307-226(367)163(89-124(9)10)291-232(373)172(99-144-105-269-119-275-144)300-219(360)155(70-77-188(262)334)284-234(375)175(102-191(265)337)303-249(390)201(127(15)16)314-211(352)150(260)92-137-44-34-31-35-45-137/h31-39,44-49,53-68,105-106,119-136,150-186,201-209,323-332H,29-30,40-43,50-52,69-104,107-118,258-260H2,1-28H3,(H2,261,333)(H2,262,334)(H2,263,335)(H2,264,336)(H2,265,337)(H2,266,338)(H,269,275)(H,270,276)(H,272,353)(H,273,355)(H,274,354)(H,277,356)(H,278,340)(H,279,341)(H,280,342)(H,281,357)(H,282,358)(H,283,369)(H,284,375)(H,285,364)(H,286,387)(H,287,388)(H,288,389)(H,289,351)(H,290,359)(H,291,373)(H,292,370)(H,293,374)(H,294,381)(H,295,366)(H,296,365)(H,297,368)(H,298,376)(H,299,371)(H,300,360)(H,301,380)(H,302,361)(H,303,390)(H,304,383)(H,305,384)(H,306,394)(H,307,367)(H,308,362)(H,309,372)(H,310,385)(H,311,391)(H,312,392)(H,313,339)(H,314,352)(H,315,378)(H,316,377)(H,317,393)(H,318,382)(H,319,386)(H,320,379)(H,321,363)(H,343,344)(H,345,346)(H,347,348)(H,349,350)(H,396,397)(H,398,399)(H4,267,268,271)/t131-,132-,133-,134+,135+,136+,150-,151-,152-,153-,154-,155-,156-,157-,158-,159-,160-,161-,162-,163-,164-,165-,166-,167-,168-,169-,170-,171-,172-,173-,174-,175-,176-,177-,178-,179-,180-,181-,182-,183-,184-,185-,186-,201-,202-,203-,204-,205-,206-,207-,208-,209-/m0/s1
1 of 1
This Item | |||
|---|---|---|---|
| biological source Saccharomyces cerevisiae | biological source - | biological source human | biological source human |
| recombinant expressed in yeast | recombinant - | recombinant expressed in yeast (proprietary host) | recombinant expressed in Saccharomyces cerevisiae |
| Quality Level 200 | Quality Level - | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form lyophilized (clear, colorless solution after reconstitution) | form powder | form solid |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| Gene Information human ... INS(3630) | Gene Information human ... INS(3630) | Gene Information human ... INS(3630) | Gene Information human ... INS(3630) |
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Storage Class
13 - Non Combustible Solids
wgk
WGK 2
flash_point_c
Not applicable
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E Roine et al.
FEBS letters, 417(2), 168-172 (1997-12-12)
Pseudomonas syringae pv. tomato DC3000 produces Hrp pili under inducing in vitro conditions. A preparation of partially purified extracellular filaments contains HrpA, flagellin and some minor contaminants. HrpA was separated from the major contaminant, the flagellin, by gel filtration to
Y Nakamura et al.
British journal of pharmacology, 123(4), 675-682 (1998-03-28)
1. The actions of troglitazone, pioglitazone, metformin and bezafibrate, agents that improve insulin-resistance, on voltage-dependent Ca2+ channels in arterial smooth muscle cells were examined by use of the conventional and nystatin-perforated whole-cell clamp methods. Single cells were freshly isolated from
S Assady et al.
Diabetes, 50(8), 1691-1697 (2001-07-27)
Type 1 diabetes generally results from autoimmune destruction of pancreatic islet beta-cells, with consequent absolute insulin deficiency and complete dependence on exogenous insulin treatment. The relative paucity of donations for pancreas or islet allograft transplantation has prompted the search for
Global Trade Item Number
| SKU | GTIN |
|---|---|
| I3536-100MG | 04061833856208 |
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After reconstitution in 0.01 M HCl, can the aliquot be frozen and for how long?
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