H9039
Synonym(s):
Chloro(protoporphyrinato)iron(III), Chlorohemin, Chloroprotoporphyrin IX iron(III), Ferriprotoporphyrin IX chloride, Hemin(chloride)
| Size/SKU | Availability | Price |
|---|
bovine
≥90%
powder
toxicology assay: suitable
1.4 M NH4OH: soluble 25 mg/mL
2-8°C
Cc1c(CCC(O)=O)c2cc3c(CCC(O)=O)c(C)c4cc5nc(cc6c(C)c(C=C)c(cc1n2)n6[Fe](Cl)n34)c(C=C)c5C
1S/C34H34N4O4.ClH.Fe/c1-7-21-17(3)25-13-26-19(5)23(9-11-33(39)40)31(37-26)16-32-24(10-12-34(41)42)20(6)28(38-32)15-30-22(8-2)18(4)27(36-30)14-29(21)35-25;;/h7-8,13-16H,1-2,9-12H2,3-6H3,(H4,35,36,37,38,39,40,41,42);1H;/q;;+3/p-3/b25-13-,26-13-,27-14-,28-15-,29-14-,30-15-,31-16-,32-16-;;
BTIJJDXEELBZFS-HXFTUNQESA-K
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| description from bovine, ≥90% | description BioXtra, from Porcine, ≥96.0% (HPLC) | description Ready-made microbial growth medium supplement, 0.5 g/L | description Iron-containing compound of porcine origin that can induce hydrogen peroxide-dependent lipid peroxidation. Reported to increase the number and size of myotubes and the frequency of myotube contractions. |
| biological source bovine | biological source Porcine | biological source - | biological source - |
| technique(s) toxicology assay: suitable | technique(s) transfection: suitable, tissue culture: suitable | technique(s) - | technique(s) - |
| assay ≥90% | assay ≥96.0% (HPLC) | assay - | assay - |
| form powder | form powder or crystals | form liquid | form solid |
| solubility 1.4 M NH4OH: soluble 25 mg/mL | solubility - | solubility - | solubility 0.1-1.0 M NaOH: soluble, 1 N NH4OH: soluble |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 15-25°C |
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11 - Combustible Solids
WGK 3
Not applicable
Not applicable
Eyeshields, Gloves, type N95 (US)
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The extinction coefficient at the absorbance maximum (613 nm) for this product is ≈ 4.14 mM⁻¹·cm⁻¹, equivalent to ≈ 4,140 M⁻¹·cm⁻¹.
Please see the link below to access a Certificate of lot interest:
https://www.sigmaaldrich.com/product/sigma/h9039#product-documentation
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This Hemin product is not Halal or Kosher certified. A Halal or Kosher certified Hemin product is not currently offered.
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This product is supplied in powder form and needs to be reconstituted. It is soluble in 1.4 M ammonium hydroxide at 25 mg/mL. Please review the product data page at: https://www.sigmaaldrich.com/product/sigma/h9039
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This product is tested for solubility at 25 mg/mL in 1.4 M NH4OH. According to a published article, this material is also soluble at 50 mg/mL in 1.0 M NaOH. Please see the link below to review this publication:
https://journals.asm.org/doi/10.1128/iai.01924-05
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Stock solutions of Hemin are typically added to medium and autoclaved at 121 °C for 15-20 minutes. Stock solutions may also be filter sterilized and added aseptically to sterile medium.
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A number of products are not included in expiration testing programs, as there is no indication to conclude they are unstable. The only date shown on the certificate of analysis will be the Release Date. For these products there is no stated Retest, Expiration or Use-by-Date Date. We recommend handling per defined conditions as printed in our product literature and website product descriptions. Products should be routinely inspected by the customer to ensure they perform as expected. For those products, only our standard warranty of 1 year from the date of shipment of products is applicable. For further information on our warranty please refer to terms and conditions of sale.
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What is the shelf life for Hemin, Product H9039?
There is no shelf life date assigned to this product. The guarantee of quality is for one year from the time of shipment for bottles stored as recommended.
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What are some applications for Hemin, Product H9039?
Hemin-agarose has reportedly been prepared for use in affinity chromatography of heme-binding proteins. See Tsutsui, K., Affinity chromatography of heme-binding proteins: Synthesis of hemin-agarose. Meth. Enzymol. 123, 331, (1986). Sigma supplies a hemin agarose, product H6390. Hemin can also be used as a component in culture media for the cultivation of bacteria, such as Leishmania tarentolae.
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How stable is Hemin, Product H9039, in solution?
Sigma-Aldrich has not evaluated the solution stability of hemin solutions. Also, we have not found any information on solution storage in the literature. We recommend that solutions be prepared fresh.
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