H7787

HIS-Select^® Spin Columns

• Synonym(s): His-Tag Spin Columns
• NACRES: NA.56
• UNSPSC Code: 41106500
• Pore size: 100 nm pore size
• Packaging: pkg of 10 ea, pkg of 50 ea

Properties

• shelf life: 2 yr at 2‑8 °C
• Quality Segment: 200
• packaging: pkg of 10 ea, pkg of 50 ea
• pore size: 100 nm pore size
• capacity: >0.5 mg/column
• storage temp.: 2-8°C

Description

General description

Kit contains 3 collection tubes for every spin column.

Application

Pre-packed and ready-to-use HIS-Select Spin Columns allow for fast and consistent purification of small-scale crude cell extracts containing histidine-tagged proteins. The spin columns contain a matrix of silica particles and uses the HIS-Select patented non-charged, hydrophilic nickel chelating linkage chemistry. HIS-Select Spin Columns are highly selective for histidine-tagged proteins and exhibit very low non-specific binding of other proteins. These convenient Spin Columns allow for fast, one-step purification for the isolation of at least 150 μg of histidine-tagged protein in less than 15 minutes.

Features and Benefits

• High Selectivity for higher purity
• Convenient and Ready-to-Use
• Consistent protein binding each use
• Compatible with CelLytic^™ cell lysis reagents

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable