H7416

IL-6 human

≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

• Synonym(s): Interleukin-6 human, IL-6
• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD00131403
• Biological source: human
• Recombinant: expressed in HEK 293 cells
• Assay: ≥95% (SDS-PAGE)
• Form: lyophilized powder
• Mol wt: dimer 26-30 kDa (glycosylated)
• Impurities: ≤1.00 EU/μg

Properties

• Product Name: Interleukin-6 human, IL-6, recombinant, expressed in HEK 293 cells, suitable for cell culture
• biological source: human
• Quality Segment: 100
• recombinant: expressed in HEK 293 cells
• assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• potency: ≤2.0 ng/mL ED₅₀
• quality: endotoxin tested
• mol wt: dimer 26-30 kDa (glycosylated)
• packaging: pkg of 10 μg
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1.00 EU/μg
• UniProt accession no.: P05231
• storage temp.: −20°C
• Gene Information: human ... IL6(3569)

Description

General description

Recombinant human Interleukin‑6 (IL‑6) is a cytokine expressed in HEK 293 cells and supplied as a lyophilized protein. IL‑6 is commonly used in mammalian cell culture research involving immune‑related cell models.

Application

Recombinant IL‑6 has been used to elucidate its role in breast cancer metastasis and in D5HS tumor sphere formation.

Biochem/physiol Actions

Interleukin-6 (IL-6) is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. IL-6 appears to be directly involved in the responses that occur after infection and injury and may prove to be as important as IL-1 and TNF-α in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. IL-6 induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor (PCT-GF), interferon-β-2 (IFN-β₂), monocyte derived human B cell growth factor, B cell stimulating factor (BSF-2), hepatocyte stimulating factor (HSF), Interleukin Hybridoma/Plasmacytoma-1 (IL-HP1). In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 also potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors.

Interleukin-6 (IL-6) is produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It induces B-cell differentiation and is a primary regulator of inflammation. IL6 regulates tumor proliferation and inhibits apoptosis. It also potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors.

Preparation Note

Human IL-6, expressed in human 293 cells, is a glycosylated monomer with an apparent molecular mass of 26-30 kDa due to glycosylation, which is absent when this cytokine is expressed in E. coli. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable