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H1270

Horse Serum

Donor herd, USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma

Synonym(s):

HS, equine sera, equine serum, horse sera, sera, serum

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71
MDL number:
MFCD00164115
Biological source:
horse serum
Origin:
USA origin
Sterility:
sterile-filtered
Form:
liquid
Shipped in:
dry ice
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biological source

horse serum

Quality Segment

500

sterility

sterile-filtered

form

liquid

composition

Hemoglobin, ≤20 mg/dL

origin

USA origin

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

impurities

≤10 EU/mL Endotoxin

shipped in

dry ice

storage temp.

−20°C

General description

Horse serum produced from adult horses is homogenous and has low levels of polyamine oxidase. It is metabolically stable and promotes cell proliferation. Horse sera is an alternate to bovine serum and is a source of growth factors and hormones.

Application

Horse Serum has been used as a supplement in culture medium in pheochromocytoma cell culture, cortical slice cultures, retinal cells and myotubes.

Preparation Note

Collected from a controlled herd.

Other Notes

Donor Horse Serum is collected from controlled donor herds located in the USA. Horse serum contains high protein and low trace metals compared to Fetal Bovine Serum. Donor Horse Serum is suitable for use in diagnostic assays, as a supplement in mycoplasma growth and assay media and for culturing hematopoietic stem cells and neuronal cells.

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This Item
12449CH0146H1138
Horse Serum Donor herd, USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma

Sigma-Aldrich

H1270

Horse Serum

Horse Serum USA origin, Donor herd, suitable for cell culture, suitable for hybridoma

Sigma-Aldrich

12449C

Horse Serum

Horse serum liquid

Sigma-Aldrich

H0146

Horse serum

Horse Serum Donor Herd, USA origin, Heat inactivated, sterile-filtered, suitable for cell culture

Sigma-Aldrich

H1138

Horse Serum

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

origin

USA origin

origin

USA origin

origin

-

origin

USA origin

biological source

horse serum

biological source

horse serum

biological source

horse serum

biological source

horse serum

sterility

sterile-filtered

sterility

sterile-filtered

sterility

non-sterile

sterility

sterile-filtered

form

liquid

form

-

form

liquid

form

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

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Storage Class

12 - Non Combustible Liquids

wgk

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ppe

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Questions

  1. Does this product (H1270) need heat inactivation?

    1 answer

    1. This product has not been heat-inactivated. For most cell culture applications heat inactivation is not necessary. If the application requires the inactivation of any heat-labile serum components, such as complement or cytokines, the product can be heat-inactivated in the lab. Alternatively, a heat-inactivated version of this product is also available.

      To heat inactivate this product:
      1. Allow serum containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.
      2. A water-filled control bottle (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C, start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum, or alter the performance of the serum.
      3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.
      4. At the end of 30 minutes remove the serum bottles from the water bath. Aliquot the serum into smaller volumes and freeze. Sigma-Aldrich does not recommend repeated thaw-freeze cycles.

      Please see the link below to review the heat-inactivated product, H1138:
      https://www.sigmaaldrich.com/product/sigma/h1138

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