G7396

β-Glucuronidase from Escherichia coli

Type IX-A, lyophilized powder, 1,000,000-5,000,000 units/g protein (30 min assay)

• Synonym(s): β-D-Glucuronide glucuronosohydrolase
• CAS Number: 9001-45-0
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-606-8
• MDL number: MFCD00199013
• EC Number: 3.2.1.31 (BRENDA, IUBMB)
• Specific activity: 1,000,000-5,000,000 units/g protein (30 min assay)

Properties

• type: Type IX-A
• Quality Segment: 300
• form: lyophilized powder
• specific activity: 1,000,000-5,000,000 units/g protein (30 min assay)
• mol wt: 290 kDa
• composition: Protein, 30-70% biuret
• shipped in: wet ice
• storage temp.: −20°C

Description

Application

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Effective in the hydrolysis of steroid glucuronides.

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Analysis Note

Contains buffer salts and stabilizer.

Other Notes

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Storage Class: 11 - Combustible Solids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, type N95 (US)
• Hazard Codes: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• WGK: WGK 1