G6378

Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)

Type XV, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)

• Synonym(s): G-6-P-DH, Zwischenferment
• CAS Number: 9001-40-5
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-602-6
• MDL number: MFCD00081656
• EC Number: 1.1.1.49 (BRENDA, IUBMB)
• Specific activity: 200-400 units/mg protein (modified Warburg-Christian)

Properties

• type: Type XV
• Quality Segment: 300
• form: lyophilized powder
• specific activity: 200-400 units/mg protein (modified Warburg-Christian)
• mol wt: 128 kDa
• purified by: crystallization
• application(s): diagnostic assay manufacturing
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Glucose 6-phosphate dehydrogenase (G-6-P-DH) is a key regulatory enzyme in the first step of the pentose phosphate pathway. G-6-P-DH is a glycoprotein with a molecular mass of 128 kDa (gel filtration).

Application

Glucose-6-phosphate dehydrogenase is used to test ketose reductase activity in developing maize endosperm.

Biochem/physiol Actions

Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway<<<17>>>.

Physical form

Lyophilized and essentially sulfate-free; contains approx. 20% sodium citrate based on dry weight

Preparation Note

Prepared from G7877

Other Notes

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NADP at pH 7.4 at 25 °C.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)