G4507

Giemsa stain

Technical grade, powder

• Synonym(s): Azure mixture sicc. Giemsa stain
• Empirical Formula (Hill Notation): C₁₄H₁₄ClN₃S
• CAS Number: 51811-82-6
• Molecular Weight: 291.80
• UNSPSC Code: 12171500
• PubChem Substance ID: 329799928
• NACRES: NA.47
• MDL number: MFCD00081642

Properties

• Product Name: Giemsa stain, technical grade, used as a blood stain
• grade: technical grade
• Quality Segment: 100
• form: powder
• color: dark greenvery dark
• mp: 300 °C (lit.)
• solubility: ethanol: 1 mg/mL
• λmax: 645-655 nm
• suitability: application for blood stain
• application(s): diagnostic assay manufacturing; hematology; histology; microbiology
• storage temp.: room temp
• SMILES string: [Cl-].CN(C)c1ccc2N=C3C=CC(=[NH2+])C=C3Sc2c1
• InChI: 1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H
• InChI key: NALREUIWICQLPS-UHFFFAOYSA-N

Description

General description

Giemsa stain is a versatile differential stain that is widely used in hematology for blood and bone marrow specimens, clinical cytology specimens, bacteriology, histological biopsies, and tumor samples. Giemsa’s staining solution is composed of methylene blue, azure, and eosin. It is a major histological stain due to its unique staining of chromatin, nuclear membranes, and cytoplasmic elements. The stain is frequently used in combination with other dye solutions: May-Grünwald’s solution for Pappenheim (MGG) and Wright-Giemsa.

Application

Giemsa stain has been used for the staining of cells and blood films.

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): dust mask type N95 (US), Eyeshields, Gloves