G4259

β-Glucuronidase from Helix aspersa (garden snail)

Type HA-4

• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-606-8
• MDL number: MFCD00130629
• EC Number: 3.2.1.31 (BRENDA, IUBMB)
• Specific activity: ≥300,000 units/g solid solid

Properties

• type: Type HA-4
• Quality Segment: 200
• form: partially purified powder
• specific activity: ≥300,000 units/g solid solid
• secondary activity: ≤7,500 units/g solid sulfatase
• solubility: H₂O: soluble 1.90-2.10 mg/mL, clear to slightly hazy
• application(s): clinical testing
• storage temp.: −20°C

Description

Application

β-glucuronidase was used in enzymic hydrolysis of tissue homogenates for liquid chromatography-electrospray ion trap mass spectrometry (LC/MSⁿ) analysis, to study the structures of degradation products of baicalin.[1]

Biochem/physiol Actions

β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption[2][3].

Other Notes

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37°C at the pH 5.0 (30 min assay).

One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• Storage Class: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• wgk: WGK 1