G4252

Glyoxalase I from Saccharomyces cerevisiae

Grade IV, buffered aqueous glycerol solution, ≥400 units/mg protein

• Synonym(s): S-Lactoyl-glutathione methylglyoxal-lyase (isomerizing)
• CAS Number: 9033-12-9
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 4.4.1.5 (BRENDA, IUBMB)
• MDL number: MFCD00131225
• Specific activity: ≥400 units/mg protein

Properties

• type: Grade IV
• form: buffered aqueous glycerol solution
• specific activity: ≥400 units/mg protein
• foreign activity: glyoxalase II ≤1%
• storage temp.: 2-8°C

Description

General description

Glyoxalase detoxification system consists of glyoxalase (GLO)-I and GLO-II. GLO-I is a cytosolic, 42 kDa, dimeric Zn²⁺ metalloenzyme.

Application

Glyoxalase I from Saccharomyces cerevisiae has been used as a standard in Glyoxalase I assay for C. dilatata females and brooded encapsulated embryos. It has also been used as a standard for calibration curve generation for quantifying glyoxalase I from brain tissues and cerebral microvessels.

Biochem/physiol Actions

Glyoxalase I is universally expressed and involved in the protection against cellular damage due to cytotoxic metabolites such as advanced glycation end products (AGEs). It is an integral component of the detoxification system, catalyzing the conversion of reactive, acyclic a-oxoaldehydes into the corresponding a-hydroxyacids in a glutathione-dependent manner.

Physical form

Solution in 50% glycerol, 0.4 M (NH₄)₂SO₄ and 0.002 M KH₂PO₄ pH 6.5

Analysis Note

Protein determined by biuret.

Other Notes

One unit will form 1.0 μmole of S-lactoylglutathione from methylglyoxal and reduced glutathione per min at pH 6.6 at 25 °C.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)