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β-Galactosidase from bovine testes

Name: β-Galactosidase from bovine testes
Brand: SIGMA

ammonium sulfate suspension, 1.0-3.0 units/mg protein (modified Warburg-Christian)

Synonym(s):

β-D-Galactoside galactohydrolase

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About This Item

CAS Number:

9031-11-2

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-864-1

MDL number:

MFCD00130623

EC Number:

3.2.1.23 (BRENDA, IUBMB)

Specific activity:

1.0-3.0 units/mg protein (modified Warburg-Christian)

Biological source:

bovine

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Properties

biological source

bovine

Quality Segment

200

form

ammonium sulfate suspension

specific activity

1.0-3.0 units/mg protein (modified Warburg-Christian)

foreign activity

α- and β-glucosidase ≤0.2%, α-fucosidase ≤0.2%, α-galactosidase ≤0.2%, α-mannosidase ≤0.2%, β-N-acetylglucosaminidase ≤1.0%

storage temp.

2-8°C

Description

Application

β-Galactosidase from bovine testes was used for coupling to Sepharose.[1]

Biochem/physiol Actions

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop[2].

Physical form

Suspension in 3.2 M (NH₄)₂SO₄, pH approx. 5.0

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl β-D-galactopyranoside to p-nitrophenol and D-galactopyranose per min at pH 4.4 at 25 °C.

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This Item	G1875	G4155	G5635
Sigma-Aldrich G4142 β-Galactosidase from bovine testes	Sigma-Aldrich G1875 β-Galactosidase from bovine liver	Sigma-Aldrich G4155 β-Galactosidase from Escherichia coli	Sigma-Aldrich G5635 β-Galactosidase from Escherichia coli
specific activity 1.0-3.0 units/mg protein (modified Warburg-Christian)	specific activity ≥0.15 units/mg protein	specific activity ≥500 units/mg protein (biuret)	specific activity ≥500 units/mg protein
biological source bovine	biological source bovine liver	biological source -	biological source -
form ammonium sulfate suspension	form lyophilized powder	form aqueous glycerol suspension	form lyophilized powder
storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 300
foreign activity α- and β-glucosidase ≤0.2%, α-galactosidase ≤0.2%, β-N-acetylglucosaminidase ≤1.0%, α-fucosidase ≤0.2%, α-mannosidase ≤0.2%	foreign activity -	foreign activity -	foreign activity -

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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