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Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle

Name: Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle
Brand: SIGMA

lyophilized powder, ≥75 units/mg protein

Synonym(s):

D-Glyceraldehyde 3-phosphate:NAD⁺ oxidoreductase (phosphorylating), GAPDH

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About This Item

CAS Number:

9001-50-7

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-609-4

MDL number:

MFCD00131203

EC Number:

1.2.1.12 (BRENDA, IUBMB)

Specific activity:

≥75 units/mg protein

Biological source:

rabbit muscle

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Properties

biological source

rabbit muscle

Quality Segment

200

form

lyophilized powder

specific activity

≥75 units/mg protein

composition

Protein, ≥80% biuret

foreign activity

3-Phosphoglyceric phosphokinase ≤0.05%, lactic dehydrogenase, myokinase, and pyruvate kinase ≤0.01%, triosephosphate isomerase ≤0.1%

shipped in

wet ice

storage temp.

−20°C

Description

General description

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a multifunctional protein. It is basically called as a glycolytic enzyme.

Application

Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle has been used:

for measurements of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity assay
to generate a linear standard curve to analyse GAPDH activity in the experimental samples collected from mice
in anti-aggregation assays
in purified GAPDH studies[1]

Biochem/physiol Actions

Glyceraldehyde-3-phosphate dehydrogenase catalyzes the conversion of glyceraldehyde-3-phosphate into D-glycerate-1,3-bisphosphate as part of the glycolysis pathway.

Glyceraldehyde-3-phosphate dehydrogenase catalyzes the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate as part of glycolysis. It has also been shown to have roles in initiation of apoptosis, transcription activation and the shuttling of ER to Golgi vesicles<<<18,New>>>.

Physical form

Lyophilized powder containing citrate buffer salts

Other Notes

One unit will reduce 1.0 μmole of 3-phosphoglycerate to D-glyceraldehyde 3-phosphate per min in a coupled system with 3-phosphoglyceric phosphokinase at pH 7.6 at 25 °C.

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This Item	G6880	P3397	P6635
Sigma-Aldrich G2267 Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle	Sigma-Aldrich G6880 α-Glycerophosphate Dehydrogenase from rabbit muscle	Sigma-Aldrich P3397 Phosphoglucomutase from rabbit muscle	Sigma-Aldrich P6635 Phosphorylase b from rabbit muscle
specific activity ≥75 units/mg protein	specific activity ≥100 units/mg protein	specific activity ≥100 units/mg protein	specific activity ≥20 units/mg protein
biological source rabbit muscle	biological source -	biological source rabbit muscle	biological source rabbit muscle
form lyophilized powder	form lyophilized powder	form ammonium sulfate suspension	form lyophilized powder
shipped in wet ice	shipped in wet ice	shipped in wet ice	shipped in -
storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 300	Quality Level 300

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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