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DMEM/F-12

Name: DMEM/F-12
Brand: SIGMA

With stable glutamine, sodium pyruvate and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

DME/Nutrient Mixture F-12 Ham, DMEM Hams F12

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.71

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Properties

Product Name

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham, With L-alanyl-L-glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Quality Segment

400

sterility

sterile-filtered, sterile

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, tested

components

phenol red: yes
stable glutamine: yes
glucose: high
HEPES: no
NaHCO₃: yes
sodium pyruvate: yes

shipped in

ambient

storage temp.

2-8°C

Description

General description

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is a 1:1 mixture. This DMEM F-12 Ham medium differs from the original formula because it is supplemented with L-alanyl-L-glutamine dipeptide. This dipeptide provides a more stable form of glutamine for use in cell cultures as glutamine alone is highly unstable in cell cultures.

Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used to culture human fibroblasts.

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This Item	D8437	D6434	D8900
Sigma-Aldrich FG4815 Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham	Sigma-Aldrich D8437 Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham	Sigma-Aldrich D6434 Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham	Sigma-Aldrich D8900 Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham
description With L-alanyl-L-glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture	description With L-glutamine, 15 mM HEPES, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture	description With 15 mM HEPES and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture	description With L-glutamine and 15 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
form liquid	form liquid	form liquid	form powder
sterility sterile, sterile-filtered	sterility sterile-filtered	sterility sterile-filtered	sterility -
components phenol red: yes stable glutamine: yes glucose: high HEPES: no NaHCO₃: yes sodium pyruvate: yes	components glucose: 3.15 g/L HEPES: yes L-glutamine: yes phenol red: yes sodium pyruvate: 0.055 g/L	components L-glutamine: no phenol red: no NaHCO₃: yes HEPES: 15 mM sodium pyruvate: 0.055 g/L glucose: 3.15 g/L	components sodium pyruvate: 0.055 g/L HEPES: 15 mM NaHCO₃: no L-glutamine: yes glucose: 3.15 g/L phenol red: yes
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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