E4643

Heparin-Binding EGF-Like Growth Factor human

Name: Heparin-Binding EGF-Like Growth Factor human
Brand: SIGMA

≥97% (SDS-PAGE and N-terminal analysis), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

Synonym(s):

hHB-EGF, HB-EGF, Heparin-Binding Epidermal Growth Factor-Like Growth Factor

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About This Item

UNSPSC Code:

12352202

NACRES:

NA.77

MDL number:

MFCD00212935

Biological source:

human

Recombinant:

expressed in baculovirus infected Sf21 cells

Assay:

≥97% (SDS-PAGE and N-terminal analysis)

Form:

lyophilized powder

Mol wt:

9.5 kDa

Key Documents

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Product Name

Heparin-Binding EGF-Like Growth Factor human, HB-EGF, recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

biological source

human

Quality Level

100

recombinant

expressed in baculovirus infected Sf21 cells

assay

≥97% (SDS-PAGE and N-terminal analysis)

form

lyophilized powder

potency

≤3 ng/mL

quality

endotoxin tested

mol wt

9.5 kDa

packaging

pkg of 50 and 250 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

Q99075

storage temp.

−20°C

Related Categories

Cell Culture & Analysis

Cell Culture Supplements & Reagents

Growth Factors and Cytokines

Molecular Biology & Functional Genomics

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Show Differences

1 of 4

This Item	H1404	E9644	P5208
Sigma-Aldrich E4643 Heparin-Binding EGF-Like Growth Factor human	Sigma-Aldrich H1404 Hepatocyte Growth Factor human	Sigma-Aldrich E9644 hEGF	Sigma-Aldrich P5208 Platelet-Derived Endothelial Cell Growth Factor human
biological source human	biological source human	biological source human	biological source human
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
assay ≥97% (SDS-PAGE and N-terminal analysis)	assay >95% (SDS-PAGE)	assay ≥97% (SDS-PAGE)	assay ≥97% (SDS-PAGE)
recombinant expressed in baculovirus infected Sf21 cells	recombinant expressed in Baculovirus infected High-5 cells	recombinant expressed in E. coli	recombinant expressed in baculovirus infected Sf21 cells
Quality Level 100	Quality Level 200	Quality Level 300	Quality Level 200
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder

Biochem/physiol Actions

Heparin-binding epidermal growth factor (HB-EGF), like BTC, is a heparin-binding EGF family member. Natural soluble HB-EGF is a 19 kDa glycoprotein of multiple forms due to heterogeneous truncations. Soluble HB-EGF is mitogenic and chemotactic for fibroblasts, keratinocytes, and smooth muscle cells but not capillary endothelial cells. Production of HB-EGF is detected in monocytes, macrophages, and vascular and aortic smooth muscle cells (SMC). HB-EGF may play a role in the pathogenesis of atherosclerosis. It may also be an effector of Jun-induced oncogenic transformation. HB-EGF binds to EGFR with high affinity and to ErbB with moderate affinity.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.

Analysis Note

The biological activity is measured by its ability to stimulate ³H-thymidine incorporation in the EGF-responsive BALB/3T3 cells.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Granulocyte-macrophage colony-stimulating factor induces expression of heparin-binding epidermal growth factor-like growth factor/diphtheria toxin receptor and sensitivity to diphtheria toxin in human neutrophils.

F Vinante et al.

Blood, 94(9), 3169-3177 (1999-11-11)

Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a widely expressed EGF superfamily member that induces mitogenic and/or chemotactic activities toward different cell types through binding to EGF receptors 1 or 4. Membrane-bound HB-EGF exerts growth activity and adhesion capabilities

Contact-dependent growth inhibition and apoptosis of epidermal growth factor (EGF) receptor-expressing cells by the membrane-anchored form of heparin-binding EGF-like growth factor.

R Iwamoto et al.

The Journal of biological chemistry, 274(36), 25906-25912 (1999-08-28)

Heparin-binding epidermal growth factor-like growth factor (HB-EGF) transduces mitogenic signals through the EGF receptor (EGFR). There are two forms of HB-EGF, the membrane-anchored form (pro-HB-EGF) and the soluble form (sHB-EGF). We studied the biological activity of pro-HB-EGF by using a

Diphtheria toxin endocytosis and membrane translocation are dependent on the intact membrane-anchored receptor (HB-EGF precursor): studies on the cell-associated receptor cleaved by a metalloprotease in phorbol-ester-treated cells.

M Lanzrein et al.

The Biochemical journal, 310 ( Pt 1), 285-289 (1995-08-15)

Preincubation of Vero cells with 1 microM phorbol 12-myristate 13-acetate (PMA) decreased the specific binding of diphtheria toxin by about 50%, whereas the toxic effect, endocytic uptake and membrane translocation were completely blocked. Toxin bound to PMA-treated cells was released

Epidermal growth factor receptor ligands are chemoattractants for normal human mesothelial cells.

U Palmer et al.

The European respiratory journal, 14(2), 405-411 (1999-10-09)

Signalling through epidermal growth factor (EGF) receptor leads to several cellular responses including cell division and cell migration. Since EGF receptors are expressed on normal mesothelial cells, this study investigated whether EGF receptor ligands act as chemoattractants on these cells.

New molecular mechanisms in cholangiocarcinoma: signals triggering interleukin-6 production in tumor cells and KRAS co-opted epigenetic mediators driving metabolic reprogramming.

Leticia Colyn et al.

Journal of experimental & clinical cancer research : CR, 41(1), 183-183 (2022-05-27)

Cholangiocarcinoma (CCA) is still a deadly tumour. Histological and molecular aspects of thioacetamide (TAA)-induced intrahepatic CCA (iCCA) in rats mimic those of human iCCA. Carcinogenic changes and therapeutic vulnerabilities in CCA may be captured by molecular investigations in bile, where

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Global Trade Item Number

SKU	GTIN
E4643-50UG	04061833603949
E4643-250UG	04061833603932

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