DNAQF

DNA Quantitation Kit, Fluorescence Assay

Quantitation of DNA using bisBenzimide

• Synonym(s): dsDNA quantification
• NACRES: NA.84
• UNSPSC Code: 41105500

Properties

• Quality Segment: 200
• usage: sufficient for 750 assays (2 ml each)
• technique(s): nucleic acid detection: suitable
• storage temp.: −20°C

Description

General description

The kit has all the reagents necessary for quantitation of DNA using the fluorescent dye bisBenzimide (Hoechst 33258). The kit allows highly sensitive, quick, and accurate determinations of double-stranded DNA from 10ng/ml to 5μg/ml, even in the presence of RNA and proteins. Fluorometry is a highly sensitive and simple method used for DNA quantitation. The fluorescent dye, bisBenzimide H 33258, which binds primarily to AT sequences in the minor groove of double-stranded DNA (dsDNA), is specific for quantitation of nanogram amounts of DNA (10 ng/ml to 10 mg/ml). When excited at 360 nm, the fluorescence emission at 460 nm of the dye increases significantly in the presence of DNA.

Application

DNA Quantitation Kit, Fluorescence Assay has been used to measure the concentration/content of DNA:

• in neonatal rat ventricular myocytes (NRVMs)
• in human mesenchymal stem cells (hMSCs)/samples for normalization of the relative alkaline phosphatase (ALP) amount
• in mouse embryonic fibroblast cell line (NIH3T3 cells)

Features and Benefits

• Specific for the quantitation of nanogram amounts of DNA
• Works well with purified preparations as well as with DNA from crude extracts containing RNA and proteins

Kit Components Only

• Detailed technical bulletin 1 ea

Safety Information

• Storage Class: 10 - Combustible liquids