CS1010 kit measures total HDAC activity in cellular, nuclear, or purified enzyme extracts.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/401/235/cs1010bul.pdf
CS1010
Histone Deacetylase Assay Kit, Fluorometric
sufficient for 100 assays (96 well plates)
Synonym(s):
HDAC Assay Kit
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About This Item
UNSPSC Code:
12161503
EC Number:
200-664-3
NACRES:
NA.84
Pricing and availability is not currently available.
usage
sufficient for 100 assays (96 well plates)
Quality Segment
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... HDAC1(3065)
Application
The Histone Deacetylase Assay Kit provides a simple method for the detection of HDAC activity based on a two-step enzymatic reaction. The substrate for this enzymatic assay is a substituted peptide with an acetylated lysine residue and a bound fluorescent group. The first step of the reaction is deacetylation of the acetylated lysine side chain by the HDAC containing sample (HeLa cell extract, purified enzyme, etc.). The second step is the cleavage of the deacetylated substrate by the developer solution and the release of the free highly fluorescent group. The measured fluorescence is directly proportional to the deacetylation activity of the sample.
Biochem/physiol Actions
Histone deacetylases (HDACs) are enzymes that remove acetyl groups from histone proteins. HDACs are important regulators of gene expression and genome function. They are implicated in a number of human disease states, namely several cancers, neurological disorders, and aging. Therefore, HDAC are relevant key targets for therapeutic intervention. Moreover, histone deacetylase inhibitors have been shown to serve as anti-tumor agents.
Features and Benefits
The kit includes all the reagents required for the fast and easy measurement of HDAC activity in cell or nuclear extracts, or with purified enzyme preparations. In addition, the kit provides HeLa cell lysate as a source of HDAC activity for inhibitor screening or as a positive control, a HDAC inhibitor (Trichostatin A), and a standard to enable activity quantitation.
Analysis Note
The Histone Deacetylase Assay Kit has been tested on HeLa, HEK 293T, NIH 3T3, and U 973 cell extracts.
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This Item | |||
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| description sufficient for 100 assays (96 well plates) | description 1 kit sufficient for 50-100 tests | description sufficient for 30 assays (1 mL), sufficient for 100 assays (200 μL) | description sufficient for 200 colorimetric or fluorometric tests |
| Gene Information human ... HDAC1(3065) | Gene Information human ... DHFR(1719) | Gene Information human ... HMGCR(3156) | Gene Information Drosophila ... Gpdh1(33824) |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level - |
| usage sufficient for 100 assays (96 well plates) | usage kit sufficient for 50-100 tests | usage sufficient for 100 assays (200 μL), sufficient for 30 assays (1 mL) | usage sufficient for 200 colorimetric or fluorometric tests |
| storage temp. −20°C | storage temp. −20°C | storage temp. −70°C | storage temp. −20°C |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
Kit Components Only
Product No.
Description
- Assay Buffer 20 mL
- Developer Solution 1.5 mL
- HDAC Inhibitor (Trichostatin A), 1 mM 50 μL
- HDAC Substrate, 20 mM 50 μL
- HeLa Cell Lysate 500 μL
- Standard (non-acetylated substrate), 4 mM 50 μL
Storage Class
10 - Combustible liquids
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
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which enzyme mainly present in this assay kit. is it only HDAC 1?
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