Mero-25

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 9100102, human lung, Mixed epithelial and spindle shaped

• UNSPSC Code: 41106514
• Biological source: human lung
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Number of chromosomes 127-140
• Morphology: Mixed epithelial and spindle shaped
• Manufacturer/tradename: ECACC

Properties

• biological source: human lung
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 09100102-DNA-5UG, pkg of vial of cells 09100102-1VL
• growth mode: Adherent
• karyotype: Number of chromosomes 127-140
• morphology: Mixed epithelial and spindle shaped
• products: Cytokeratins 7, 8, 18 and 19. Epithelial membrane antigen (EMA)
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Application

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods

Biochem/physiol Actions

A human mesothelioma cell line derived from a malignant mesothelioma. Asbestos exposure

Mero-25 was derived from autopsy tumour material from a male patient aged >60 with a history of asbestos exposure

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12
D16S539: 11,12
D5S818: 11,12
D7S820: 9
THO1: 8
TPOX: 9
vWA: 16,17

Preparation Note

Hams F10 + 15% FCS + 2mM Glutamine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Population doubling approx 48 hours

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.