C4129

α-Chymotrypsin from bovine pancreas

Type II, lyophilized powder, ≥40 units/mg protein

• Synonym(s): α-chymotrypsin A and B, alpha-chymotrypsin
• CAS Number: 9004-07-3
• UNSPSC Code: 12352204
• eCl@ss: 42010112
• EC Number: 232-671-2
• NACRES: NA.54
• MDL number: MFCD00130481
• EC Number: 3.4.21.1 (BRENDA, IUBMB)
• Specific activity: ≥40 units/mg protein

Properties

• type: Type II
• Quality Segment: 300
• form: lyophilized powder
• specific activity: ≥40 units/mg protein
• mol wt: 25 kDa
• composition: protein, ≥85%
• color: white to off-white
• UniProt accession no.: P00767
• application(s): diagnostic assay manufacturing
• storage temp.: −20°C
• Gene Information: cow ... CTRB1(618826)

Description

Application

The enzyme from Sigma has been used to study the structure-function relationship in glycosylated α-chymotrypsin using immobilized metal-ion affinity chromatography (IMAC) and immobilized metal-ion affinity capillary electrophoresis (IMACE).

Biochem/physiol Actions

α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. The pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca²⁺ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, 10 mM Cu²⁺ and Hg²⁺.

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.

Preparation Note

Produced from 3× crystallized chymotrypsinogen

Analysis Note

Protein determined by E1%/280

Other Notes

One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.

View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer

Safety Information

• pictograms: GHS08,GHS07,GHS09
• signalword: Danger
• hcodes: H302,H315,H319,H334,H335,H400
• pcodes: P261 - P264 - P273 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves