B7542

Maleimide Activated BSA

• Synonym(s): Maleimide Activated Bovine Serum Albumin
• NACRES: NA.32
• UNSPSC Code: 12352111
• Biological source: bovine
• Form: lyophilized powder
• Technique(s): ELISA: suitable, bioconjugation: suitable
• Concentration: 5—6 mg/mL protein

Properties

• biological source: bovine
• Quality Segment: 200
• sterility: non-sterile
• form: lyophilized powder
• concentration: 5—6 mg/mL protein
• technique(s): ELISA: suitable, bioconjugation: suitable
• UniProt accession no.: P02769
• storage temp.: −20°C
• Gene Information: bovine ... ALB(280717)

Description

General description

Maleimide Activated BSA is provided in lyophilized, ready to-use form, eliminating the need for time-consuming activation and purification steps of the carrier protein. Maleimide Activated BSA contains approximately 5-15 maleimide groups for each BSA molecule.. The conjugation reaction with a cysteine containing peptide is performed at pH 6.6-7.0. The extent of conjugation can then be determined by a colorimetric assay with Ellman′s reagent) to detect unreacted thiol groups in the cysteine-containing peptide.

Application

Maleimide Activated BSA has been used in peptide conjugation.

Maleimide-activated BSA (mBSA) has reactive maleimide groups on its surface available for conjugation with a cysteine-containing peptide or a thiol-containing hapten. The reaction of the maleimide group and the thiol group proceeds rapidly and selectively under mild coupling conditions (pH 6.5-7.5) to yield a stable, covalently linked peptide-protein conjugate that may be used in immunization protocols or in antibody screening methods.

Physical form

Each vial contains 5 mg of Maleimide Activated BSA lyophilized from 10 mM sodium phosphate buffer, pH 6.6, with 0.115 mM NaCl, 1 mM EDTA, and 40 mM sucrose as stabilizer.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable