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B3133

Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride

trypsin substrate, chromogenic, ≥99% (TLC), powder, suitable for substrate for trypsin

Synonym(s):

L-BAPA, L-BAPNA, BANI

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About This Item

Empirical Formula (Hill Notation):
C19H22N6O4 · HCl
CAS Number:
Molecular Weight:
434.88
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
244-505-6
MDL number:
Beilstein/REAXYS Number:
4081878
NACRES:
NA.32
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Product Name

Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride, ≥99% (TLC), suitable for substrate for trypsin

Quality Segment

assay

≥99% (TLC)

form

powder

solubility

acetone: water (1:1): 50 mg/mL

suitability

suitable for substrate for trypsin

storage temp.

−20°C

SMILES string

Cl[H].NC(=N)NCCC[C@H](NC(=O)c1ccccc1)C(=O)Nc2ccc(cc2)[N+]([O-])=O

InChI

1S/C19H22N6O4.ClH/c20-19(21)22-12-4-7-16(24-17(26)13-5-2-1-3-6-13)18(27)23-14-8-10-15(11-9-14)25(28)29;/h1-3,5-6,8-11,16H,4,7,12H2,(H,23,27)(H,24,26)(H4,20,21,22);1H/t16-;/m0./s1

InChI key

DEOKFPFLXFNAON-NTISSMGPSA-N

General description

Chromogenic substrate for trypsin and other proteolytic enzymes.
Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride is a substrate for trypsin.

Application

Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride has been used as a substrate:
  • for chymotrypsin and trypsin activity assay in enzymatic extract (EE) of M. tenellum and pancreatic tissue homogenates
  • in trypsin and papain standard curve generation
  • in serine protease and fibrinogenolytic assays of eupolytin1 protein

Other Notes

Similar to B3279, but produced for Sigma.

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This Item
B4875B3279B4500
assay

≥99% (TLC)

assay

≥98%

assay

≥98% (HPLC)

assay

≥97% (HPLC)

solubility

acetone: water (1:1): 50 mg/mL

solubility

DMSO: 50 mg/mL (colorless to dark green-yellow to dark brown-yellow)

solubility

acetone: water (1:1): 40.00-51.00 mg/mL, clear, colorless to yellow

solubility

water: 50 mg/mL, clear, colorless

suitability

suitable for substrate for trypsin

suitability

-

suitability

-

suitability

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

powder

form

powder

form

powder

form

powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C


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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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