B1520

Anti-Bovine Albumin antibody produced in rabbit

whole antiserum

• Synonym(s): Anti-Albumin, bovine serum (BSA)
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00162166
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ELISA (i), QPA
• Citations: 25

Properties

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: whole antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• contains: 15 mM sodium azide
• species reactivity: bovine
• technique(s): indirect ELISA: 1:200,000, quantitative precipitin assay: 3.0 mg/mL
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: bovine ... Alb(280717)

Description

General description

Serum albumin in humans is encoded by the gene mapped to human chromosome 4q13.3. Serum albumin is one of the abundant proteins in the blood and has a molecular weight of 66 kDa.
Anti-Bovine Serum Albumin (BSA) was developed in rabbit using purified BSA from Cohn Fraction V as the immunogen. The antiserum has been treated to remove lipoproteins. Strong reactivity with BSA has been determined by immunoelectrophoresis (IEP).

Application

Anti-Bovine Albumin antibody produced in rabbit may be used for ELISA at a working dilution of 1:200,000. The antibody was used for in vitro labeling of albumin present in mammary tissue extract of lactating cow.
It has also been used for the detection of albumin in nanoparticle using scanning transmission electron microscope.[1]

Biochem/physiol Actions

Albumin is the most abundant circulating proteins present in the serum that binds and transports long-chain fatty acid anions, detoxifies unconjugated bilirubin and transports thyroid and steroid hormones.
Human serum albumin as a transporter, is considered as drug delivery vehicle for treating human diseases. Post-translational modifications like glycation and cysteinylation affects the binding functionality of serum albumin. High albumin protein diet has influence on hyperalbuminemia. Low levels of albumin leads to hypoalbuminemia, associated with decreased production and distribution. Hypoalbuminemia is a risk factor for the development of cardiovascular disease.

Physical form

Rabbit Anti-BSA is provided as a liquid containing 15 mM sodium azide as preservative.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable