A6684

Monoclonal Anti-Albumin antibody produced in mouse

clone HSA-11, ascites fluid

• Synonym(s): Anti-AFP
• MDL number: MFCD00162390
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: HSA-11, monoclonal
• contains: 15 mM sodium azide
• species reactivity: gibbon, baboon, human, rhesus monkey
• should not react with: turkey, feline, hamster, pigeon, rat, marmoset, pig, bovine, canine, donkey, guinea pig, rabbit, chicken, sheep, goat
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; indirect ELISA: 1:10,000; western blot: 1:5,000
• isotype: IgG2a
• UniProt accession no.: P02768
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ALB(213)

Description

General description

Monoclonal Anti-Albumin (mouse IgG2a isotype) is derived from the HSA-11 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Albumin, the major protein produced by the liver, represents more than half the total protein in human serum. Many other body fluids also contain albumin.

Serum albumin is the major protein component of blood and is produced in the liver. It regulates colloid osmotic blood pressure, transportation of a variety of substances, blood pH and provides an endogenous source of amino acids. Monoclonal anti-albumin antibody (diluted 1:100) can be used in double staining to localize hepatocytes differentiated from human umbilical mesenchymal stem cells (HUMSCs) . Offering specificity for human serum albumin, this product is ideal for identification of human blood stains or other body fluids (e.g., seminal fluid, milk, urine, saliva, vaginal secretions). Mouse monoclonal anti-albumin antibody reacts specifically with denatured and reduced human serum albumin and cross reacts with rhesus, gibbon and baboon serum albumins. The product has shown no cross reactivity with bovine, cat, chicken, dog, donkey, goat, guinea pig, hamster, marmoset, pig, pigeon, rabbit, rat, sheep, or turkey serum albumin or with chicken and turkey egg albumin.

Specificity

The antibody is specific for human albumin in serum and plasma and recognizes an epitope resistant to reduction or denaturation.

Immunogen

Human serum albumin

Application

Monoclonal Anti-Albumin antibody produced in mouse has been used in:

• immunofluorescence
• staining
• double staining
• enzyme-linked immunosorbent assay (ELISA)
• immunoblot
• immunohistochemistry

Biochem/physiol Actions

Albumin is a transport protein that binds a broad range of ligands such as fatty acids, bilirubin, hemin, drugs, amino acids and ions . Albumin may also function as a zinc carrier protein and consequently regulate physiological processes .

High levels of albumin are associated with dehydration. Albumin turnover is seen in infants with iron deficiency anemia. Oxidative damage of albumin is associated with advanced liver disease.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable