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Apyrase from potatoes

Name: Apyrase from potatoes
Brand: SIGMA

ATPase ≥200 units/mg protein, lyophilized powder

Synonym(s):

Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase

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About This Item

CAS Number:

9000-95-7

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-569-8

MDL number:

MFCD00130542

EC Number:

3.6.1.5 (BRENDA, IUBMB)

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Properties

type

Grade VII

Quality Segment

300

form

lyophilized powder

ATPase activity

≥200 units/mg protein

secondary activity

≥50 % of base activity ADPase

composition

Protein, 25-60%

solubility

H₂O: soluble 1.0 mg/mL

application(s)

diagnostic assay manufacturing

foreign activity

Acid Phosphatase ≤2% of base activity

storage temp.

−20°C

Description

Application

At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1).
Reaction: ATP → ADP+P_i → AMP+2P_i.

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation [1].

Biochem/physiol Actions

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis [2]. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.

Packaging

Sold on the basis of ATPase units.

Physical form

Partially purified, lyophilized powder containing potassium succinate buffer salts.

Preparation Note

Derived from red potato

Other Notes

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

This grade has a low ATPase/ADPase ratio.

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This Item	SRE0019	A7646	A6410
Sigma-Aldrich A6535 Apyrase from potatoes	Sigma-Aldrich SRE0019 Apyrase from potatoes	Sigma-Aldrich A7646 Apyrase from potatoes	Sigma-Aldrich A6410 Apyrase from potatoes
form lyophilized powder	form lyophilized powder	form lyophilized powder (partially purified)	form lyophilized powder
application(s) diagnostic assay manufacturing	application(s) diagnostic assay manufacturing	application(s) diagnostic assay manufacturing	application(s) -
solubility H₂O: soluble 1.0 mg/mL	solubility H₂O: soluble 1.0 mg/mL	solubility H₂O: soluble 10 mg/mL, slightly hazy	solubility H₂O: soluble 1.0 mg/mL
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
foreign activity Acid Phosphatase ≤2% of base activity	foreign activity Acid Phosphatase ≤2% of base activity	foreign activity Acid Phosphatase ≤5% of base activity	foreign activity Acid Phosphatase ≤2% of base activity
Quality Level 300	Quality Level 400	Quality Level 200	Quality Level 200

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pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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