A2765

Azocasein

protease substrate, chromogenic, powder

• Synonym(s): Sulfanilamide-azocasein
• CAS Number: 102110-74-7
• UNSPSC Code: 12352204
• NACRES: NA.32
• MDL number: MFCD00130592

Properties

• Product Name: Azocasein, protease substrate
• biological source: bovine
• Quality Segment: 200
• form: powder
• technique(s): ligand binding assay: suitable
• solubility: water: 5 mg/mL, clear, orange to very deep orange
• ε (extinction coefficient): ≥25 at 440 nm in 0.1 M NaOH at 1%
• storage temp.: 2-8°C

Description

General description

Azocasein is a chromogenic derivative of casein. Protease degrades azocasein to yield TCA-soluble azopeptides with high UV-absorbance. This azocasein assay is widely employed to estimate the protease production by bacterial fermentation on synthetic substrates from glucose and inorganic salts.

Application

Azocasein is a nonspecific protease substrate. Hydrolysis of the casein releases the azo dye into the media where it is detected by absorbance at 440 nm.

Azocasein is an inflammatory agent that is used to induce amyloid A amylooidosis in experimental animals.

Azocasein has been used as a substrate for determination of protease activity.

Other Notes

View more information on azocasein protease assay at www.sigma-aldrich.com/enzymeexplorer

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)