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A0157

Ascorbate Oxidase from Cucurbita sp.

lyophilized powder, 1,000-3,000 units/mg protein

Synonym(s):

L-Ascorbate:oxygen oxidoreductase, Ascorbase

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100 units

Available to ship TODAYfromMILWAUKEE

$65.30
250 units

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$131.00
1000 units

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$361.00

About This Item

CAS Number:
9029-44-1
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-852-6
MDL number:
MFCD00130560
EC Number:
1.10.3.3 (BRENDA, IUBMB)
Specific activity:
1,000-3,000 units/mg protein
Biological source:
plant (Cucurbita spp.)

$65.30

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biological source

plant (Cucurbita spp.)

Quality Segment

200

form

lyophilized powder

specific activity

1,000-3,000 units/mg protein

storage temp.

2-8°C

General description

Ascorbate oxidase is a homodimeric enzyme, which comprises 552 amino acid residues per subunit (zucchini). It corresponds to a molecular mass of 70kDa per subunit. This enzyme is mainly found in plants, fungi and eubacteria.

Application

Ascorbate Oxidase from Cucurbita sp. has been used:
  • as a supplement in the culture medium for differentiation into osteoblasts
  • to oxidize ascorbic acid producing monodehydroascorbate in monodehydroascorbate reductase assay
  • in determining ascorbate (AsA) and dehydroascorbate (DHA) concentrations

Ascorbate oxidase, from Cucurbita sp., may be used to study oxidative stress and heat stress response and tolerance. Ascorbate oxidase, from Sigma, has been used in ascorbic acid assays to study the heat stress response of Arabidopsis [1].

Biochem/physiol Actions

Ascorbate oxidase (AO) participates in cell growth by regulating reduction/oxidation (redox) of the apoplast. This enzyme can synthesize the oxidative molecule dehydroascorbate acid (DHA) in the apoplast. It is also involved in cell elongation and enlargement development.
Ascorbate oxidase converts ascorbic acid to dehydroascorbic acid. It is highly specific for L-ascorbic acid and a few analogs. Ascorbate oxidase exists as a dimer and has a molecular weight of approximately 140 kDa.

Physical form

Lyophilized powder containing buffers and sucrose as stabilizer.

Other Notes

One unit will oxidize 1.0 μmole of L-ascorbate to dehydroascorbate per min at pH 5.6 at 25 °C.

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This Item
189724P410510736619001
Ascorbate Oxidase from Cucurbita sp. lyophilized powder, 1,000-3,000 units/mg protein

Sigma-Aldrich

A0157

Ascorbate Oxidase from Cucurbita sp.

Ascorbate Oxidase, Cucurbita sp. Native ascorbate oxidase from Cucurbita species. Catalyzes the oxidation of ascorbioxidation of ascorbic acid to dehydroascorbic acid. Suitable for eliminating interference of ascorbic acid in clinical assays and for determination of ascorbic acid.

Sigma-Aldrich

189724

Ascorbate Oxidase, Cucurbita sp.

Pyruvate Oxidase from Aerococcus sp. lyophilized powder, ≥35 units/mg protein (biuret)

Sigma-Aldrich

P4105

Pyruvate Oxidase from Aerococcus sp.

Ascorbate Oxidase Spatula from Cucurbita species

Roche

10736619001

Ascorbate Oxidase Spatula

specific activity

1,000-3,000 units/mg protein

specific activity

≥1500 U/mg, ≥300 units/mg material

specific activity

≥35 units/mg protein (biuret)

specific activity

17 units

biological source

plant (Cucurbita spp.)

biological source

-

biological source

bacterial (Aerococcus spp.)

biological source

plant (Cucurbita species)

form

lyophilized powder

form

lyophilized

form

lyophilized powder

form

solid

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

-

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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