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60142

Potassium chloride solution

BioUltra, Molecular Biology, ~1 M in H2O

Synonym(s):

KCl, potassium chloride

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About This Item

Linear Formula:
KCl
CAS Number:
Molecular Weight:
74.55
UNSPSC Code:
12352302
NACRES:
NA.26
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
1711999
Grade:
Molecular Biology
Form:
liquid (Clear)
Solubility:
water: soluble
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grade

Molecular Biology

Quality Level

product line

BioUltra

form

liquid (Clear)

concentration

~1 M in H2O

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.001% total nitrogen (N)

pH

5.0-8.0 (25 °C, 1 M in H2O)

solubility

water: soluble

density

1.04 g/mL at 20 °C

anion traces

sulfate (SO42-): ≤10 mg/kg

cation traces

Al: ≤1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Na: ≤20 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg

λ

1 M in H2O

UV absorption

λ: 260 nm Amax: 0.02, λ: 280 nm Amax: 0.01

SMILES string

[Cl-].[K+]

InChI

1S/ClH.K/h1H;/q;+1/p-1

InChI key

WCUXLLCKKVVCTQ-UHFFFAOYSA-M

General description

Potassium is an intracellular cation with just 2% of total body K+ inhabiting the extracellular fluid. The distribution of K+ between the intracellular and extracellular space is analyzed by the action of several transport pathways like Na+-K+-ATPase, the Na+-K+-2Cl-cotransporter NKCC1, the four KCCs, and a plethora of K+ channels. Also, skeletal muscle comprises almost 75% of total body potassium and elicits considerable influence on extracellular K+. Potassium chloride is consumed orally to prevent or correct potassium depletion caused by diuretic use or other conditions. It is used as a food supplement and salt substitute.

Application

Potassium chloride solution has been used as a component of:
  • multiple annealing and looping based amplification cycles (MALBAC) lysis buffer to digest proteins for single-cell multiomics sequencing[1]
  • single-cell tagged reverse transcription (STRT) buffer, and STRT-RT buffer for single-cell RNA-sequencing[2]
  • phosphate buffer saline (PBS) to wash transfected cells[3]


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This Item
60135P954160137
form

liquid (Clear)

form

liquid

form

powder

form

liquid

solubility

water: soluble

solubility

water: soluble

solubility

water: soluble (355 g/l at 25 °C (77 °F))

solubility

water: soluble

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

grade

Molecular Biology

grade

reagent grade

grade

Molecular Biology

grade

-

density

1.04 g/mL at 20 °C

density

1.120 g/cm3 at 20 °C

density

1.98 g/mL at 25 °C (lit.)

density

1.13 g/mL at 20 °C

impurities

DNases, none detected, insoluble matter, passes filter test, proteases, none detected, RNases, none detected, ≤0.001% total nitrogen (N), phosphatases, none detected

impurities

insoluble matter, passes filter test, ≤0.001% total nitrogen (N)

impurities

-

impurities

-


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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves



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Meyler's Side Effects of Drugs (2016)
Transport of Sodium, Chloride, and Potassium
Brenner and Rector's The Kidney E-book (2019)
Takanobu Kato et al.
Nature protocols, 1(5), 2334-2339 (2007-04-05)
Hepatitis C virus (HCV) infection causes chronic liver disease and is a worldwide health problem. Despite ever-increasing demand for knowledge on viral replication and pathogenesis, detailed analysis has been hampered by a lack of efficient viral culture systems. We isolated



Global Trade Item Number

SKUGTIN
60142-100ML-F04061832659442
60142-500ML-F04061832659459

Questions

1–3 of 3 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Why is it approximately (~) 1 M? What is the tolerance in concentration around 1 M?

    1 answer
    1. The certificate of analysis for this product (a sample of which can be seen in the 'Documentation' section of the product page) indicates that the specification range is 0.95 to 1.10 M.

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