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Merck

137265

HEPES sodium salt

EMPROVE® EVOLVE

Synonym(s):

HEPES, Sodium Salt, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt

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About This Item

Empirical Formula (Hill Notation):
C8H17N2NaO4S
CAS Number:
Molecular Weight:
260.29
UNSPSC Code:
12161700
MDL number:

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InChI key

RDZTWEVXRGYCFV-UHFFFAOYSA-M

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

SMILES string

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

biological source

synthetic

product line

EMPROVE® EVOLVE

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, microbial, and trace metals; tested

pH

6.8-8.2

useful pH range

6.8-8.2

pKa (25 °C)

7.5

pKa 

7.5

suitability

suitable for manufacturing use

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

storage temp.

2-30°C

Quality Level

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This Item
H3784H7006H3662
form

powder

form

powder

form

powder

form

solution

biological source

synthetic

biological source

-

biological source

-

biological source

-

impurities

Endotoxin, microbial, and trace metals; tested

impurities

endotoxin and total aerobic microbial count, tested

impurities

-

impurities

Bioburden, tested, DNase, RNase. Protease, Nickase, free, endotoxin, tested, <5 ppm Heavy metals (as Pb)

suitability

suitable for manufacturing use

suitability

suitable for component for culture media, suitable for molecular biology

suitability

suitable for mass spectrometry

suitability

-

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

foreign activity

DNase, RNase, NICKase, protease, none detected

foreign activity

-

foreign activity

-

technique(s)

cell culture | mammalian: suitable

technique(s)

activity assay: suitable, cell culture | mammalian: suitable

technique(s)

HPLC: suitable

technique(s)

cell culture | mammalian: suitable

Application

HEPES Sodium is a biological buffer often referred to as a “Good′s” buffer. HEPES Sodium, used in conjunction with HEPES, is the most commonly used biological buffer formulation since the pKa of the buffer is 7.31 which corresponds to the physiological pH of 7.36. HEPES Sodium is considered to be non-toxic to culture cell lines, highly water soluble and provides high-solution clarity.HEPES Sodium is used in cell culture media, biopharmaceutical buffer formulations (both upstream and downstream) and diagnostic reagent formulations.

Legal Information

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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